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Eukaryotic origin-dependent DNA replication in vitro reveals sequential action of DDK and S-CDK kinases.

(Submitter supplied) Initiation of eukaryotic DNA replication requires temporal separation of helicase loading from helicase activation and replisome assembly. Using an in vitro assay for eukaryotic origin-dependent replication initiation, we investigated the control of these events. After helicase loading, we found that the Dbf4-dependent Cdc7 kinase (DDK) initially drives origin recruitment of Sld3 and the Cdc45 helicase-activating protein. more...
Organism:
Saccharomyces cerevisiae
Type:
Genome binding/occupancy profiling by genome tiling array
Platform:
GPL5991
4 Samples
Download data:
GEO (TXT)
Series
Accession:
GSE29646
ID:
200029646

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