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    Transcriptome changes in splicing inhibited cells

    (Submitter supplied) In eukaryotes, U1 small nuclear ribonucleoprotein (snRNP) forms spliceosomes in equal stoichiometry with U2, U4, U5 and U6 snRNPs; however, its abundance in human far exceeds that of the other snRNPs. Here we used antisense morpholino oligonucleotide to U1 snRNA to achieve functional U1 snRNP knockdown in HeLa cells, and identified accumulated unspliced pre-mRNAs by genomic tiling microarrays. In addition to inhibiting splicing, U1 snRNP knockdown caused premature cleavage and polyadenylation in numerous pre-mRNAs at cryptic polyadenylation signals, frequently in introns near (<5 kilobases) the start of the transcript. more...
    Organism:
    Homo sapiens
    Type:
    Expression profiling by genome tiling array
    Platform:
    GPL4914
    3 Samples
    Download data:
    GEO (BAR, CEL)
    Series
    Accession:
    GSE24179
    ID:
    200024179

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