Display Settings:

Format

Send to:

Choose Destination
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

    A native chromatin purification system for epigenomic profiling in C. elegans

    (Submitter supplied) We describe an in vivo chromatin purification system for genome-wide epigenetic profiling in C. elegans. In this system, we coexpressed the E. coli biotin ligase enzyme (BirA), together with the C. elegans H3.3 gene fused to BioTag, a 23-amino acid peptide serving as a biotinylation substrate for BirA, in vivo in worms. We developed methods to isolate chromatin under different salt extraction conditions, followed by affinity purification of biotinylated chromatin with streptavidin and genome-wide profiling with microarrays. more...
    Organism:
    Caenorhabditis elegans
    Type:
    Genome binding/occupancy profiling by genome tiling array
    Platform:
    GPL7098
    18 Samples
    Download data:
    GEO (BEDGRAPH, GFF, PAIR, TXT)

      Supplemental Content

      Recent activity

      Your browsing activity is empty.

      Activity recording is turned off.

      Turn recording back on

      See more...
      Write to the Help Desk