GEO DataSets

Analysis of hepatocytes isolated from chimeric mouse livers repopulated with human hepatocytes (c-heps) or from normal human livers (h-heps). Results provide insight into the potential use of chimeric mice as a relevant model for human liver biomedical experiments.

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by array, transformed count, 2 species, 23 tissue sets

Platform:

GPL570

Series:

GSE33846

32 Samples

Download data: CEL

(Submitter supplied) We generated chimeric mice with livers that were predominantly repopulated with human hepatocytes. Hepatocytes were isolated from the chimeric mouse livers and their gene expressions were compared with hepatocytes isolated from normal human livers . Cluster and principal components analyses showed that gene expression profiles of hepatocytes from the chimeric mice and those from normal human livers were extremely closed. more...

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4327

Platform:

GPL570

32 Samples

Download data: CEL

(Submitter supplied) We performed microarray experiments to examine gene expression in human tissues. This data was used for comparison with our humanized mouse study (GEO ID GSE33846) and threshold determination of our tiling array data (GEO ID GSE18490, public in the near future). A total of 22 tissues (bone marrow, cerebellum, colon, cortex, fetal brain, heart, kidney, liver, lung, pancreas, prostate, salivary gland, skeletal muscle, small intestine, spinal cord, spleen, stomach, testes, thymus, thyroid, trachea and uterus) and 2 cell lines (HeLa and SH-SY5Y) were examined.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

24 Samples

Download data: CEL

(Submitter supplied) We generated h-hepatocyte chimeric mice with livers that were predominantly repopulated with h-hepatocytes in a h-growth hormone (GH)-deficient state. Using microarray profiles, comparison between h-hepatocytes from h-GH-treated and untreated mice identified 14 GH-up-regulated and four GH-down-regulated genes, including IGF-1, SOCS2, NNMT, IGFLS, P4AH1, SLC16A1, and SRD5A1, and FADS1 and AKR1B10, respectively.

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by array

Platform:

GPL570

14 Samples

Download data: CEL

(Submitter supplied) Generation of human fibroblast-derived hepatocytes capable of extensive proliferation, as evidenced by significant liver repopulation of mice. Unlike current protocols for deriving hepatocytes from human fibroblasts, ours did not generate iPSCs, but shortcut reprogramming to pluripotency to generate an induced multipotent progenitor cell (iMPC) stage from which endoderm progenitor cells (iMPC-EPCs) and subsequently hepatocytes (iMPC-Heps) could be efficiently differentiated. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

11 Samples

Download data: CEL

(Submitter supplied) We established a protein-based artificial transcription system and successfully engineered human hepatocyte-like cells from human umbilical cord-derived mesenchymal stem cells (MSC-UCs). We utilized four artificial transcription factors (ATFs) that targeted hepatocyte nuclear factor (HNF)1α, HNF3γ, HNF4α and GATA4 (4F), and found that treatment with 4F for 5 days converted MSC-UCs to hepatocyte-like cells (4F-Heps). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL21185

9 Samples

Download data: TXT

(Submitter supplied) The molecular determinants of a healthy human liver cell phenotype remain largely uncharacterized. In addition, the gene expression changes associated with activation of primary human hepatic stellate cells, a key event during fibrogenesis, remain poorly characterized. Here, we provide the transriptomic profile underpinning the healthy phenotype of human hepatocytes, liver sinusoidal endothelial cells (LSECs) and quiescent hepatic stellate cells (qHSCs) as well as activated HSCs (aHSCs) We assess the transcriptome for purified, non-cultured human hepatocytes, liver sinusoidal cells (LSECs) and quiescent hepatic stellate cells (qHSCs) as well as culture-activated HSCs (aHSCs).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13667

11 Samples

Download data: CEL

(Submitter supplied) The human liver contains multiple cell types whose epigenetic patterns are undetermined. We examined the promoter methylome of purified and uncultured hepatic stellate cells (HSCs), hepatocytes (HEPs) and liver sinusoidal endothelial cells (LSECs), by methylated DNA immunoprecipitation (MeDIP) and array hybridization. Uncultured HSCs, LSECs and Heps show ~7000-8000 methylated promoters, with 60-70% similarity between all cell types. more...

Organism:

Homo sapiens

Type:

Methylation profiling by genome tiling array

Platform:

GPL15802

10 Samples

Download data: PAIR, TXT

(Submitter supplied) The role of PPARα in gene regulation in mouse liver is well characterized. However, less is known about the effect of PPARα activation in human liver. The aim of the present study was to better characterize the impact of PPARα activation on gene regulation in human liver by combining transcriptomics with the use of hepatocyte humanized livers. To that end, chimeric mice containing hepatocyte humanized livers were given an oral dose of 300 mg/kg fenofibrate daily for 4 days. more...

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by array

Platform:

GPL11532

6 Samples

Download data: CEL

(Submitter supplied) To elucidate the effect of a prostaglandin D2 receptor DP1 agonist BW245C on concanavalin A (ConA) hepatitis, we performed DNA microarray using SurePrint G3 Mouse GE 8x60K Microarray. Mice were treated with ConA for 3 or 24 hr with vehicle or BW245C . Livers were collected, homogenized, and subjected to total RNA extraction.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13912

25 Samples

Download data: TXT

(Submitter supplied) We have established culture systems to generate liver progenitor cells (LPCs), liver sinusoidal endothelial cells (LSECs), and hepatic stellate cells (HSCs) from hiPSCs. Then, we established co-culture system of hiPSC-derived liver cells and performed RNA-seq of hiPSC-derived liver model.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

6 Samples

Download data: TXT

(Submitter supplied) We have established culture systems to generate liver sinusoidal endothelial cells (LSECs) and hepatic stellate cells (HSCs) from hiPSCs. To characterize gene expression profiling in hiPSC-derived LSECs and HSCs, transcriptome analysis was performed.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL20844

4 Samples

Download data: TXT

(Submitter supplied) Background/Aim: Legalon SIL (SIL) is a chemically hydrophilized version of silibinin that has exhibited hepatoprotective and antiviral effectiveness against hepatitis C virus (HCV) in patients leading to viral clearance in combination with ribavirin. To elucidate the incompletely understood mode of action of SIL against HCV, we studied viral response kinetics and cellular gene expression during SIL treatment in the absence of an adaptive immune response in uPA-SCID chimeric mice with humanized livers. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13915

9 Samples

Download data: TXT