Display Settings:

Format
Items per page
Sort by

Send to:

Choose Destination
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information

    Results: 20

    1.

    TGF-beta/Smad2/3 signaling directly regulates several miRNAs in mouse ES Cells and early embryos

    (Submitter supplied) Purpose: We aimed to identify miRNAs which are induced by the Activin/Nodal effectors, P-Smad2/3, in order to further our understanding of how P-Smad2/3 controls downstream gene expression in mouse ES cells to regulate crucial biological processes. Methods: We used a previously developed Tetracycline-On (Tet-On) system (TAG1) to manipulate the levels of P-Smad2/3 in mouse ES cells and performed an Illumina deep-sequencing screen to identify miRNAs which followed the P-Smad2/3 pathway. more...
    Organism:
    Mus musculus
    Type:
    Non-coding RNA profiling by high throughput sequencing
    Platform:
    GPL9250
    3 Samples
    Download data:
    GEO (TXT), SRA SRP014760
    Series
    Accession:
    GSE39994
    ID:
    200039994
    2.

    TGF-beta-induced gene expression data and Smad2/3 binding sites of HaCaT keratinocytes

    (Submitter supplied) Smad2/3 are transcription factors that engage in TGF-beta-induced transcription. Here we analyzed the effect of identified Smad2/3 binding sites to transcription. We used expression microarrays to compare the Smad2/3 binding sites identified by ChIP-chip to TGF-beta-induced gene expressions. Keywords: time course We also examined the effect of either ETS1/TFAP2A/SMAD2/SMAD3 siRNAs on TGF-beta-induced gene expression change.
    Organism:
    Homo sapiens
    Type:
    Expression profiling by array; Genome binding/occupancy profiling by genome tiling array
    Platforms:
    GPL570 GPL7026
    20 Samples
    Download data:
    GEO (CEL)
    Series
    Accession:
    GSE11710
    ID:
    200011710
    3.

    Gene expression profiling in murine Smad-deficient CD4+ T cells stimulated with TGF-b

    (Submitter supplied) TGF-b is an important pleiotropic cytokine with potent immunoregulatory properties. Although many previous reports have been proposed for the immunoregulatory functions of TGF-b on T cells, such as the suppression of cell proliferation, cytokine production and cytokine signaling, as well as the induction of apoptosis, it is not well elucidated whether the each effect of TGF-b on T cells is dependent on Smad signaling or Smad-independent other signaling pathways. more...
    Organism:
    Mus musculus
    Type:
    Expression profiling by array
    Platform:
    GPL1261
    8 Samples
    Download data:
    GEO (CEL, CHP)
    Series
    Accession:
    GSE19601
    ID:
    200019601
    4.

    Activin/Nodal signaling in mouse embryonic stem cells

    (Submitter supplied) Members of the transforming growth factor (TGF)-β superfamily play essential roles in the pluripotency, self-renewal, and differentiation of embryonic stem cells. While bone morphogenic proteins maintain pluripotency of undifferentiated mouse ES cells, the role of Activin/Nodal signaling is less clear. To determine the target genes of Activin/Nodal-Smad2 signaling in undifferentiated embryonic stem cells, changes in gene expression were examined following stimulation with recombinant Activin (2 hours) or after inhibition of Activin/Nodal with SB431542 (24 hours) using defined media culture conditions with LIF and 20 ng/mL BMP4. more...
    Organism:
    Mus musculus
    Type:
    Expression profiling by array
    Platform:
    GPL1261
    6 Samples
    Download data:
    GEO (CEL)
    Series
    Accession:
    GSE17879
    ID:
    200017879
    5.

    mRNA expression data from primary untreated neuroblastoma tumour samples

    (Submitter supplied) The miR-17-92 microRNA cluster is often activated in cancer cells, but the identity of its targets remains largely elusive. Here we examined the effects of activation of the entire miR-17-92 cluster on global protein expression in neuroblastoma cells. In this dataset we deposit global mRNA expression data obtained form primary neuroblastoma tumour cells. This data was used to demonstrate negative correlation between TGFB target gene expression and expression of the miR-17-92 cluster.
    Organism:
    Homo sapiens
    Type:
    Expression profiling by array
    Platform:
    GPL5175
    40 Samples
    Download data:
    GEO (CEL)
    Series
    Accession:
    GSE21713
    ID:
    200021713
    6.

    HNF4A-binding sites in HepG2 hepatoblastoma cells treated with TGF-beta

    (Submitter supplied) Specific regulation of target genes by transforming growth factor-β (TGF-β) in a given cellular context is determined in part by transcription factors and cofactors that interact with the Smad complex. In the present study, we determined Smad2 and Smad3 (Smad2/3) binding regions in the promoters of known genes in HepG2 hepatoblastoma cells, and compared them to those in HaCaT epidermal keratinocytes to elucidate the mechanisms of cell type- and context-dependent regulation of transcription induced by TGF-β. more...
    Organism:
    Homo sapiens
    Type:
    Genome binding/occupancy profiling by high throughput sequencing
    Platform:
    GPL9115
    2 Samples
    Download data:
    GEO (BED, WIG)
    Series
    Accession:
    GSE28845
    ID:
    200028845
    7.

    Cell-type-specific target selection by combinatorial binding of Smad2/3 and hepatocyte nuclear factor 4-alpha in HepG2 cells

    (Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
    Organism:
    Homo sapiens
    Type:
    Expression profiling by array; Genome binding/occupancy profiling by genome tiling array; Genome binding/occupancy profiling by high throughput sequencing
    Platforms:
    GPL5082 GPL9115 GPL570
    11 Samples
    Download data:
    GEO (BAR, BED, CEL, WIG)
    Series
    Accession:
    GSE28798
    ID:
    200028798
    8.

    Smad2/3 binding sites in HaCaT, HepG2, and Hep3B cells determined by an Affymetrix promoter array

    (Submitter supplied) Specific regulation of target genes by transforming growth factor-β (TGF-β) in a given cellular context is determined in part by transcription factors and cofactors that interact with the Smad complex. In the present study, we determined Smad2 and Smad3 (Smad2/3) binding regions in the promoters of known genes in HepG2 hepatoblastoma cells, and compared them to those in HaCaT epidermal keratinocytes to elucidate the mechanisms of cell-type- and context-dependent regulation of transcription induced by TGF-β. more...
    Organism:
    Homo sapiens
    Type:
    Genome binding/occupancy profiling by genome tiling array
    Platform:
    GPL5082
    3 Samples
    Download data:
    GEO (BAR, BED, CEL)
    Series
    Accession:
    GSE28797
    ID:
    200028797
    9.

    Expression data of the human hepatoblastoma cell line HepG2 treated with TGF-beta

    (Submitter supplied) Smad2/3 are transcription factors that engage in TGF-beta-induced transcription. We determined and analyzed HepG2 and Hep3B-specific Smad2/3 binding sites by ChIP-chip. We used expression microarrays to compare the Smad2/3 and HNF4alpha binding sites identified by ChIP-chip or ChIP-seq, respectively, to TGF-beta-induced gene expressions.
    Organism:
    Homo sapiens
    Type:
    Expression profiling by array
    Platform:
    GPL570
    6 Samples
    Download data:
    GEO (CEL)
    Series
    Accession:
    GSE28590
    ID:
    200028590
    10.

    Graded Nodal/Activin Signaling Governs ES Cell Fate Decisions via Differential Recruitment of Phospho-Smad2 to Oct4 and Distinct Target Gene Subsets

    (Submitter supplied) Nodal and Activin are morphogens of the TGFbeta superfamily of signaling molecules that direct differential cell fate decisions in a dose- and distance-dependent manner. During early embryonic development the Nodal/Activin pathway is responsible for the specification of mesoderm, endoderm, node and mesendoderm. In contradiction to this drive towards cellular differentiation, the pathway also plays important roles in the maintenance of self-renewal and pluripotency in embryonic and epiblast stem cells. more...
    Organism:
    Mus musculus
    Type:
    Expression profiling by array
    Platform:
    GPL6103
    16 Samples
    Download data:
    GEO (TXT)
    Series
    Accession:
    GSE23239
    ID:
    200023239
    11.

    Smad3 regulates the miR-200 family in gastric cancer

    (Submitter supplied) To find potential microRNA links between Smad3 and E-cadherin, we characterized the microRNA profiles of two gastric cancer cell lines: SNU484-LPCX, which does not express Smad3, and SNU484-Smad3, in which Smad3 is overexpressed. We found that miR-200 families, among other differentially expressed miRNAs, are overexpressed in SNU484-Smad3. Through subsequent studies, including silencing of Smad3 in SNU484-Smad3 and expression profiling of epithelial-mesenchymal markers and ZEB1/2, known repressors of E-cadherin, we found that Smad3 regulates miR-200 families at the transcriptional level, which regulate ZEB 1/2, known transcriptional repressors of E-cadherin, at the post-transcriptional level. more...
    Organism:
    Homo sapiens
    Type:
    Non-coding RNA profiling by high throughput sequencing
    Platform:
    GPL9052
    2 Samples
    Download data:
    GEO (TXT)
    Series
    Accession:
    GSE27977
    ID:
    200027977
    12.

    TGF-b signaling and response

    (Submitter supplied) This work evaluates new and specific therapeutic targets and molecular diagnostic markers connected to the TGF-b response system in chronic fibrotic diseases. Keywords: other
    Organism:
    Mus musculus
    Type:
    Expression profiling by array
    Platform:
    GPL362
    74 Samples
    Download data:
    GEO
    Series
    Accession:
    GSE579
    ID:
    200000579
    13.

    AECOM 9K mouse array; 6M-8M print series

    (Submitter supplied) cDNA spotted microarray; 8,976 cDNAs from Incyte GEM1 mouse cloneset; chip layout in 4(tips)X51(columns)X44(rows).
    Organism:
    Mus musculus
    1 Series
    74 Samples
    Download data:
    GEO
    Platform
    Accession:
    GPL362
    ID:
    100000362
    14.

    Smad2 and 3 transcription factors control muscle mass in adulthood

    (Submitter supplied) Loss of muscle mass occurs in a variety of diseases including cancer, chronic heart failure, AIDS, diabetes and renal failure, often aggravating pathological progression. Preventing muscle wasting by promoting muscle growth has been proposed as a possible therapeutic approach. Myostatin is an important negative modulator of muscle growth during myogenesis and myostatin inhibitors are attractive drug targets. more...
    Organism:
    Mus musculus
    Type:
    Expression profiling by array
    Platform:
    GPL1261
    3 Samples
    Download data:
    GEO (CEL, CHP)
    Series
    Accession:
    GSE15397
    ID:
    200015397
    15.

    Gene expression profiling of human gliomas and human glioblastoma cell lines

    (Submitter supplied) To identify signaling pathways that are differentially regulated in human gliomas, a microarray analysis on 30 brain tumor samples (12 primary glioblastomas (GBM), 3 secondary glioblastomas (GBM-2), 8 astrocytomas (Astro) and 7 oligodendrogliomas (Oligo)) and on 5 glioblastoma cell lines (LN018, LN215, LN229, LN319 and BS149) was performed. Normal brain tissue (NB) and normal human astrocytes (NHA) were used as a control. more...
    Organism:
    Homo sapiens
    Type:
    Expression profiling by array
    Datasets:
    GDS4467 GDS4468
    Platform:
    GPL570
    45 Samples
    Download data:
    GEO (CEL)
    Series
    Accession:
    GSE15824
    ID:
    200015824
    16.
    Full record GDS4468

    Glioblastoma cell lines: LN018, LN215, LN229, LN319 and BS149

    Analysis of 5 glioblastoma cell lines (LN018, LN215, LN229, LN319 and BS149). Results provide insight into molecular mechanisms underlying glioblastoma multiforme and other aggressive brain cancers.
    Organism:
    Homo sapiens
    Type:
    Expression profiling by array, count, 5 cell line sets
    Platform:
    GPL570
    Series:
    GSE15824
    10 Samples
    Download data:
    GEO (CEL)
    DataSet
    Accession:
    GDS4468
    ID:
    4468
    17.
    Full record GDS4467

    Primary and secondary brain tumors: glioblastomas, astrocytomas and oligodendrogliomas

    Analysis of primary glioblastomas (GBM), astrocytomas, oligodendrogliomas and secondary GBM brain tumors. MNK1 kinase upregulation observed in primary GBM brain tumors. Results identifiy signaling pathways differentially regulated in gliomas.
    Organism:
    Homo sapiens
    Type:
    Expression profiling by array, count, 5 cell type, 8 other, 3 tissue sets
    Platform:
    GPL570
    Series:
    GSE15824
    35 Samples
    Download data:
    GEO (CEL)
    DataSet
    Accession:
    GDS4467
    ID:
    4467
    18.

    TGF-beta/Smad3-dependent gene expression analysis in naive CD4 T cells

    (Submitter supplied) Investigation of transcript level modulation in unstimulated and TGF-beta treated (with or without superimposed T-cell receptor and CD28 stimulation) naive CD4 T cells from wild type or Smad3-deficient littermate mice. Smad3 is a critical signaling molecule and transcription factor downstream of TGF-beta and mediates several of the TGF-beta dependent tolerogenic effects in T cells. This study was undertaken to unveil the transcriptionnal program controled by the TGF-b/Smad3 axis.
    Organism:
    Mus musculus
    Type:
    Expression profiling by array
    Platform:
    GPL9899
    18 Samples
    Download data:
    GEO (PAIR, TXT)
    Series
    Accession:
    GSE40494
    ID:
    200040494
    19.

    The transcriptional regulators TAZ and YAP direct Transforming Growth Factor-beta-induced tumorigenic phenotypes in breast cancer cells

    (Submitter supplied) Uncontrolled Transforming growth factor-beta (TGFβ) signaling promotes aggressive metastatic properties in late-stage breast cancers. However, how TGFβ-mediated cues are directed to induce late-stage tumorigenic events is poorly understood, particularly given that TGFβ has clear tumor suppressing activity in other contexts. Here we demonstrate that the transcriptional regulators TAZ and YAP (TAZ/YAP), key effectors of the Hippo pathway, are necessary to promote and maintain TGFβ-induced tumorigenic phenotypes in breast cancer cells. more...
    Organism:
    Homo sapiens
    Type:
    Expression profiling by array
    Platform:
    GPL15034
    12 Samples
    Download data:
    GEO (CEL)
    Series
    Accession:
    GSE56445
    ID:
    200056445
    20.

    Myocardin-like Protein (MKL)-2 Regulates TGF-β Signaling in Embryonic Stem Cells and the Developing Vasculature

    (Submitter supplied) Signal transduction from the extracellular matrix to the arterial wall plays a critical role during development of the vasculature. We now report the discovery of a Myocardin-like Protein (MKL)2/TGF-β signaling pathway that is required for maturation and stabilization of the vasculature. Mkl2-/- null embryos exhibit profound derangements in the tunica media leading to aneurismal dilation, dissection and hemorrhage. more...
    Organism:
    Mus musculus
    Type:
    Expression profiling by array
    Platform:
    GPL6246
    5 Samples
    Download data:
    GEO (CEL)
    Series
    Accession:
    GSE38316
    ID:
    200038316

      Display Settings:

      Format
      Items per page
      Sort by

      Send to:

      Choose Destination

      Supplemental Content

      db=gds|term=|query=1|qty=2|blobid=NCID_1_965731247_130.14.18.34_9001_1406351361_1107528074_0MetA0_S_MegaStore_F_1|ismultiple=true|min_list=5|max_list=20|def_tree=20|def_list=|def_view=|url=/Taxonomy/backend/subset.cgi?|trace_url=/stat?
         Taxonomic Groups  [List]
      Tree placeholder
          Top Organisms  [Tree]

      Find related data

      Recent activity

      Your browsing activity is empty.

      Activity recording is turned off.

      Turn recording back on

      See more...
      Write to the Help Desk