Mouse fibroblast, 3T3-L1 cell line, embryo
ESX000001203 - Experiment
Summary
Chromatin factor GR assayed by ChIP-seq in Mus musculus, strain SWR, treatment with insulin, dexamethasone and iso-butyl-methyl-xanthine stimules differentiation of 3T3-L1 cells into adipocytes. Full differentiation occurs between 7-14 days after treatment. From Steger, et al. 2010.
Keywords: chromatin factor, GR
Genome track
| Track name: | 3T3-L1_6hr_GR |
| Genome release: | NCBI37 (mm9) |
| Accession number: | NA000001682.1 |
| File type: | WIG |
| File: | ESX000001203.wig.gz |
| Note: |
1 - Data were not submitted in wig format. Tracks were generated by NCBI from submitted raw data. See how. |
DownloadView on Genome
Experimental details
| Feature: | GR |
| Antibody: | Santa Cruz sc-1004 |
| Technique: | ChIP-seq |
| Instrument: | Illumina Genome Analyzer II |
| Assay Type: | chromatin factor |
Sample
Mouse fibroblast, 3T3-L1 cell line, embryo
Mus musculus, strain SWR, treatment with insulin, dexamethasone and iso-butyl-methyl-xanthine stimules differentiation of 3T3-L1 cells into adipocytes. Full differentiation occurs between 7-14 days after treatment. Features assayed include Cebpb, GR. From Steger, et al. 2010.
Study
Propagation of Adipogenic Signals through an Epigenomic Transition State
In this study, the glucocorticoid receptor (GR), CCAAT/enhancer binding protein b (CEBPb), and histone H3 acetylation are examined by ChIP- sequencing in 3T3-L1 cells before, during and after differentiation into adipocytes.
Data submission
Propagation of Adipogenic Signals through an Epigenomic Transition State
The transcriptional mechanisms by which temporary exposure to developmental signals instigates adipocyte differentiation are unknown. During early adipogenesis, we find transient enrichment of the glucocorticoid receptor (GR), CCAAT/enhancer binding protein b (CEBPb), p300, mediator subunit 1, and ...
University of Pennsylvania. Public on: May 20 2010
GSM544722 - GEOSRA
Reference
Propagation of adipogenic signals through an epigenomic transition state.
Steger DJ, et al. Genes Dev. 2010 May 15;24(10):1035-44. - Similar
PMID: 20478996 -
Citation