Pol II - Human T lymphocyte, blood, CD4+ activated (18hr.)
ESX000000193 - Experiment
Summary
Chromatin factor Pol II assayed by ChIP-seq in Homo sapiens, activation was performed by addition of anti-CD3/anti-CD28 antibody coated beads. From Barski, et al. 2009.
Keywords: chromatin factor, Pol II
Genome track
| Track name: | HS_CD4_Act_18h_ Pol II |
| Genome release: | GRCh37 (hg19) |
| Accession number: | NA000001388.1 |
| File type: | WIG |
| File: | ESX000000193.wig.gz |
| Note: |
1 - Data were not submitted in wig format. Tracks were generated by NCBI from submitted raw data. See how. |
DownloadView on Genome
Experimental details
| Feature: | Pol II |
| Antibody: | Abcam 5408 |
| Technique: | ChIP-seq |
| Instrument: | Illumina Genome Analyzer |
| Assay Type: | chromatin factor |
Sample
Human T lymphocyte, blood, CD4+ activated (18hr.)
Homo sapiens, activation was performed by addition of anti-CD3/anti-CD28 antibody coated beads. Features assayed include H2A.Z, H3K27me1, H3K27me3, H3K36me3, H3K4me1, H3K4me3, H3K79me2, H3K9me1, H4K20me1, Pol II. From Barski, et al. 2009.
Study
Chromatin Poises miRNA- and Protein-coding Genes for Expression
ChIP-seq analysis of several histone modifications, RNA polII and the histone variant H2A.Z in activated CD4+ T-lymphocytes.
Data submission
Chromatin Poises miRNA- and Protein-coding Genes for Expression
Chromatin modifications have been implicated in regulation of gene expression. While association of certain modifications with expressed or silent genes has been established, it remains unclear how changes in chromatin environment relate to changes in gene expression. In this report, we used ChIP-S...
Laboratory of Molecular Immunology/National Heart Lung and Blood Institute/National Institutes of Health. Public on: Aug 10 2009
GSM417829 - GEOSRA
Reference
Chromatin poises miRNA- and protein-coding genes for expression.
Barski A, et al. Genome Res. 2009 Oct;19(10):1742-51. - Similar
PMID: 19713549 -
Citation