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    Mouse embryonic stem cell, V6.5 cell line, undifferentiated, passage 10-15

    ESX000000069 - Experiment

    Summary

    Input control assayed by ChIP-seq in Mus musculus, strain 129SvJae x C57BL/6. From Mikkelsen, et al. 2007.

    Keyword: H3

    Genome track

    Track name:ES_H3_ChIPSeq
    Genome release:
    File type:WIG
    File:
    Note:

    1 - Data were not submitted in wig format. Tracks were generated by NCBI from submitted raw data. See how.

    View on Genome

    Experimental details

    Feature: H3
    Antibody: Abcam 1791
    Technique: ChIP-seq
    Instrument: Illumina Genome Analyzer
    Assay Type: input control

    Study

    Genome-wide maps of chromatin state in pluripotent and lineage-committed cells

    Histone modifications are examined in a variety of mouse cell types (undifferentiated and differentiated). Embryonic stem cells, embryonic fibroblasts, and neural progenitor cells are examined in this study.

    Data submission

    Genome-wide maps of chromatin state in pluripotent and lineage-committed cells

    Genome-wide maps of chromatin state (H3K4me3, H3K9me3, H3K27me3, H3K36me3, H4K20me3) in pluripotent and lineage-committed cells We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in mammalian cells. By obtaining over four ...

    Broad Institute. Public on: Jul 25 2008

    GSM307624 - GEOSRA

    Reference

    Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA.

    Goren A, et al. Nat Methods. 2010 Jan;7(1):47-9. - Similar

    Genome-wide maps of chromatin state in pluripotent and lineage-committed cells.

    Mikkelsen TS, et al. Nature. 2007 Aug 2;448(7153):553-60. - Similar

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