1FGG


Conserved Protein Domain Family
GlcAT-I
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cd00218: GlcAT-I 
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Beta1,3-glucuronyltransferase I (GlcAT-I) is involved in the initial steps of proteoglycan synthesis.
Beta1,3-glucuronyltransferase I (GlcAT-I) domain; GlcAT-I is a Key enzyme involved in the initial steps of proteoglycan synthesis. GlcAT-I catalyzes the transfer of a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region of trisaccharide Gal-beta-(1-3)-Gal-beta-(1-4)-Xyl of proteoglycans. The enzyme has two subdomains that bind the donor and acceptor substrate separately. The active site is located at the cleft between both subdomains in which the trisaccharide molecule is oriented perpendicular to the UDP. This family has been classified as Glycosyltransferase family 43 (GT-43).
Links
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Statistics
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PSSM-Id: 132995
Aligned: 23 rows
Threshold Bit Score: 236.038
Created: 4-Sep-2001
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site
Evidence:
  • Structure:1FGG; Human Glcat-I with bound to substrate and cofactor
    - View structure with Cn3D
  • Comment:donor UDP; acceptor Gal-beta-(1-3)-Gal-beta-(1-4)-Xyl;
  • Comment:glu, asp, glu may dictate specifity for the acceptor substrate
  • Comment:his may orient glucoronic acid for catalysis or stabilize the transition state
  • Comment:nearby glu may be the catalytic base in the transfer reaction
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1              #                             #                                        
1FGG_A      1 MTIYVVTPTYARLVQKAELVRLSQTLSLVP-RLHWLLVEDAegPTPLVSGLLAASGLLFTHLvvltpkaqrlregepgwv 79  human
BAB10957  152 RTVIVVTPTYVRTFQALHLTGVMHSLMLVPyDLVWIVVEAGg-ITNETASFIAKSGLKTIHLgfd-------------qk 217 thale cress
CAI93181  103 RPVIAVTPTTTSALQVPSLITLAHTLRLVDgPLRWIVVEPGh-RTDAVAAVLARSNLDFIHLvas--------------d 167 Hordeum vulgare
AAD45998  141 KLLIVVTPTYNRAMQAYYLNRVAQTLRLVEsPVLWIVVEGNv-ASFETSEILRKTGVMYRHLvckrn---------mtsi 210 thale cress
CAI94898  111 KLLIVVTPTYNRAFQAFYLNRLAHTLKLVPpPLLWIVVEMPa-QTMETAELLRKTGVMYRHLvcekn---------vtdi 180 white spruce
EDQ83892    7 KLLIIVTPTYVRPFGAYYLTRLAHTLKLVPpPLLWLVVEMHy-QSLETAQLLRETGIMYRHLvcdkn---------ltnv 76  Physcomitrella pat...
CAE11883   73 KLLIIVTITSVRPQQAYYLNRLAHVLKAVQaPLLWLVVEWPe-QSYETAEILRSSGVMYRHLicrkn---------ttsv 142 maize
CAE11882  116 VVTTTRSAPGDRRRRRGELLRLAHTLRLVRpPVVWTVVEPAa-DAPATAEVLRGTGVMYRHLafkped-------nftta 187 Zea mays
Q9VTG7    231 PPLYIITPTYRRPEQLAELTRLGYTLKHVV-NLLWLVIEDAnkTNPLVGHTLDRIGVPYEYMvapmpeky----kqtkka 305 fruit fly
NP_493160  13 RTIIVVTPTYKRITRLPDMIRLANTLAHVK-NLHWIVVEDGygIVPAVRELLEKTNLSYTYMahkta----------kgy 81  nematode

Feature 1                                             ####                                    
1FGG_A     80 hPRGVEQRNKALDWLRgrggavggekdppppgtQGVVYFADDDNTYSRELFEEMRWt-rGVSVWPVGLVGg--------- 149 human
BAB10957  218 mPNTWEDRHKLETKMRlhalrv-----vrekklDGIVMFADDSNMHSMELFDEIQTv-kWFGALSVGILAhsgnadelss 291 thale cress
CAI93181  168 gASTARLRMHALREVRke-------------kmDGVVVFADENGILRTELFDEAQKv-kSVGALPVGILGedeg------ 227 Hordeum vulgare
AAD45998  211 kDRGVHQRNTALEHIElh-------------klDGIVYFADDDNIYSLELFQSLRQi-sRFGTWPVAMLAqsk------- 269 thale cress
CAI94898  181 kDRGTHQRNTALAHIEqh-------------qlDGIVYFADDDNVYTLELFEQLRKi-rRFGTWPVGMLQhnk------- 239 white spruce
EDQ83892   77 kDRAASQRNTALAHIEhh-------------elDGIVYFADDGNMYTLELFEQMRNi-tRFGTWLVGILApgk------- 135 Physcomitrella pat...
CAE11883  143 rKIAVCQRNNAIYHVKrh-------------hlDGIMHFADEERSYSADVFEEMQKi-rRFGSWPVAIHVgtk------- 201 maize
CAE11882  188 dAEAHVQRNAALAHVEkh-------------rlAGVLHFADAAGVYDTHFFEEIRQi-eAFGAWPVATVSage------- 246 Zea mays
Q9VTG7    306 kPRGVSNRNRGLEYLReh-------------atEGVLYFADDDNTYDISIFEQMRYi-sKVAMWPVGLVTk--------- 362 fruit fly
NP_493160  82 pTRGWYQRTMALRYIRsswsk-------ilrehDAVVYFADDDNAYDVRLFTDYIRnvnTLGVWAVGLVGg--------- 145 nematode

Feature 1                      #                #                            #    #           
1FGG_A    150 --------------lRFEGPQVQ-DGRVVGFHTAWEP-------------------------SRPFPVDMAGFAVALPLL 189 human
BAB10957  292 ilkneqgknkekpsmPIQGPSCNsSEKLVGWHIFNTQpyakktav----------yidekapVMPSKMEWSGFVLNSRLL 361 thale cress
CAI93181  228 -----------tkesFLQAPACDeAGKLVGYHVSDETllpaa----------------rsdmLLSTRLEWAGFVLNARVL 280 Hordeum vulgare
AAD45998  270 ------------nkaILEGPVCN-GSQVIGWHTNEKSk-----------------------rLRRFHVDMSGFAFNSTIL 313 thale cress
CAI94898  240 ------------snaILEGPVCN-GSQVIGWHTNENSk-----------------------rLCRFHVNNSGFAFNSTIL 283 white spruce
EDQ83892  136 ------------srvVFEGPVCE-GEKVIGWHTSDRSk-----------------------rLRRFHVDMSGFAFNSTML 179 Physcomitrella pat...
CAE11883  202 ------------yraVLEGPICK-GNRVMGWHTVQTVqkk--------------------ssTRRFPIGFSAFAFNSTMM 248 maize
CAE11882  247 ------------kkvTVEGPLCS-ASEVVGWFSRDLDddgttrsvaydrreadldpaaagtrARTTTIGVSGFAFNSSIL 313 Zea mays
Q9VTG7    363 --------------tGVSSPIIQ-AGKLVGYYDGWIG-------------------------GRKYPVDMAGFAVSVKFL 402 fruit fly
NP_493160 146 --------------aIVEAPKVV-NHKVTGFNVRWSP-------------------------NRRFAVDMAGFAINLKYI 185 nematode

Feature 1                                   #                              #     
1FGG_A    190 LDKPn-----AQFDst--------apRGHLESSLLSHLVd----pKDLEPRAANCTRVLVWHTRTEK 239 human
BAB10957  362 WKESlddkpaWVKDlsl-------ldDGYAEIESPLSLVkd---pSMVEPLGSCGRRVLLWWLRVEA 418 thale cress
CAI93181  281 WDGAaer-peWVRDld--------avDGGAHLDSPLALVtd---aGQVEPLAKCAQAALAWSLRSDA 335 Hordeum vulgare
AAD45998  314 WDPKr-----WRRPfshptrqldtvkEGFQETSFIEQVVad---eSEMEGVPPACSSILNWHLHLDA 372 thale cress
CAI94898  284 WDPQk-----WGRPtselirqsdtdkDGLQDTKFIEQLVad---eREMEGLAHGCSKAMVWHLHLEA 342 white spruce
EDQ83892  180 WDPRr-----WKRPtlepirqldsikESSQQTSFIEQLVpd---eSYMEGRPPGCLKIMVWHLQLEA 238 Physcomitrella patens subsp. pa...
CAE11883  249 WDPQr-----WNRPpmdsvmvhsggrGGLQESRFIEKLVkn---eRQVEGLPDNCSRVMVWNFNLEP 307 maize
CAE11882  314 WDPEr-----WGRPass------lpdTSQDSIKFVQEVVle--drAKLRGIPSGCSQVMVWQYSDMP 367 Zea mays
Q9VTG7    403 KERPn-----AQMPf----------kPGYEEDGFLRSLApl--ddAEIELLADECRDILTWHTQTKK 452 fruit fly
NP_493160 186 LNSD------AVFGkdc------krgEGAPETCLIEDIGldlediKPFGYDATKVQDIMVWHTKTSP 240 nematode

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