Conserved Protein Domain Family
PTS_2-RNA

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pfam01885: PTS_2-RNA 
RNA 2'-phosphotransferase, Tpt1 / KptA family
Tpt1 catalyzes the last step of tRNA splicing in yeast. It transfers the splice junction 2'-phosphate from ligated tRNA to NAD, to produce ADP-ribose 1"-2"-cyclic phosphate. This is presumed to be followed by a transesterification step to release the RNA. The first step of this reaction is similar to that catalyzed by some bacterial toxins. E. coli KptA and mouse Tpt1 are likely to use the same reaction mechanism.
Statistics
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PSSM-Id: 460371
Aligned: 220 rows
Threshold Bit Score: 91.4469
Created: 21-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
ESS28608           887 LIKKLNFILRHGAPL----------FKLPMREDGYVRIRELLE----LDCMRS-----VSWEEIYLVVAS-NFKRRYEic 946 
EFJ11883            24 LGRALLKVLRQPVLH----------PNLEMRPDGFCKLEELLEldfmTK--SGvplssHTAAEAIKAVDK-LTKKLAKmv 90  
EMR66208            59 IAKAVAFVLKRSVPEseaseddeeaGHLHSDAEGWVSVAHVLE----HPKIADl---gATLKDLQRIVST-SNKSRFAlr 130 
Q2HCC9              60 IAKALMFVVKRAIQKedvee-dedgEYLVADPEGWISLADVLT----HSRISAl---gATIEDIQRVVGT-AAKSRFDlr 130  Chaetomi...
jgi:MYCTH_2307835   60 IAKALMFVVKRAIQKedvee-gdegEYLVADPEGWVSVADVLA----HSRLSAl---gATFDDIRRVIAN-APKPRFDlr 130 
XP_001593651        61 VTRALTKLLKRFSEEdnel--eegtEKFVASAEGWLSCEKVLK------NLETl---dLTLLELRKILQSpSSKPRFTlk 129 
EPE29374            60 ISKALTFVLKRTIKEgekqe-egteEKLVADADGWVDCEDVLE----KENLKSl---eVTFEELHSVVTS--PKSRFAvk 129 
EKD14451            61 ISKALTWLLKRTIEAgeeq--gegeEKLVADSDGWVGCQE-LK----RSNLASl---eVTFPELKAFVESpASKSRFGlk 130 
XP_007853293        60 ITRQMSWLLRHAAQS----------QGIRIRPDGYVRVNDLLSyylfKR---------YNMKSLQELLRY-DPKHRFEfk 119 
EDR05686           131 FGKNAGWLLRHGARH----------LGFPIRPDGFMRLQDALHfstlNR---------HTEETFLEHAIN-DPPQRFEvk 190 
ESS28608           947 ydptepehvgetgdlcvldelrnpvsqetmppavpatddasdlsfsptshhdadrkaarreeatkntnttssrtedrlhs 1026
EFJ11883            91 de------------------------------------------------------------------------------ 92  
EMR66208           131 qrpea--------------------------------------------------------------------------- 135 
Q2HCC9             131 lvsdte-------------------------------------------------------------------------- 136  Chaetomi...
jgi:MYCTH_2307835  131 kasnad-------------------------------------------------------------------------- 136 
XP_001593651       130 sissse-------------------------------------------------------------------------- 135 
EPE29374           130 lksdsd-------------------------------------------------------------------------- 135 
EKD14451           131 lrpdakeve----------------------------------------------------------------------- 139 
XP_007853293       120 eeegl--------------------------------------------------------------------------- 124 
EDR05686           191 kerdyf-------------------------------------------------------------------------- 196 
ESS28608          1027 passfpssfhststsssasfsssadsskaryesaafpkdaslgekslfsslrgrqaregdasggsggsgfcdvpafpssa 1106
EFJ11883               --------------------------------------------------------------------------------     
EMR66208               --------------------------------------------------------------------------------     
Q2HCC9                 --------------------------------------------------------------------------------      Chaetomi...
jgi:MYCTH_2307835      --------------------------------------------------------------------------------     
XP_001593651           --------------------------------------------------------------------------------     
EPE29374               --------------------------------------------------------------------------------     
EKD14451           140 ------------------------------------------------------------------------------de 141 
XP_007853293           --------------------------------------------------------------------------------     
EDR05686               --------------------------------------------------------------------------------     
ESS28608          1107 apelandrWLLRATQ------GHT--Irq-----vAsdllL--RSI--CDPGeLPV-CVHGTFLKNWMAIR-SAGLSRM- 1166
EFJ11883            93 -----tgaLYIGALQepvapnGET-------------------EQLfqKIEKsp--vCVYGTSKQELEEIM-KNGLTAS- 144 
EMR66208           136 -taedataYQVRPNS------KRNivSep----------vLvgEQFttETED-LPEfIVYETSYQGYPLILaSGGIKRAg 197 
Q2HCC9             137 gnaeepasWQISRIA------SKEtvTspt----------pvgDKLtaESPN-IPEfVVYETSYQRYPLILaLGAITRAp 199  Chaetomi...
jgi:MYCTH_2307835  137 aepeepaaWQVSRIT------HKEttAspv----------pvgDKLtaDSPD-LPEfVIYETSYQRYPLLLaLGAITRAp 199 
XP_001593651       136 tesdspsdYLIRLN-------TPStpTtitpsnisMa--sL--ELItmSSAD-LPDfVVYETSYSNYPLILaSGGIRKAg 203 
EPE29374           136 aeeadesdYLIRMIAsaipstTPSatTq-----------kL--TPLstTTED-LPDlIVYETSYANYPLILqSGGIKRAg 201 
EKD14451           142 eeaasasvYLIKANP------SPAgpAspt--satTp--kY--TPIslTTED-LPDfVVYETSYPNYPLILaSGGIKRAg 208 
XP_007853293       125 --wrgskeWLVRHQG------GHT--IkgvsssakR----I--TSFpkS--Sa----AIYCTDIQRWKTFS-EHGIWSEr 181 
EDR05686           197 -svsdvevWWVRAKE------GHD--Ipg---------vyVdrKRLfpDTTLt---eVYFRTGFDQWEKIK-KQGIPPA- 253 
ESS28608          1167 -HRNHIHFAPGLpsecgVVSGMRR-SS--------D---VAIYVNVHLAM-KEG-VRFYVSNNNVILTEG 1221
EFJ11883           145 -SSNPMYLVMAVpd-aeIASSMRN-SS--------E---VLITVNFQKAR-KDRsIEFYTSENGLIFSSG 199 
EMR66208           198 -GSTHLSFSP--------VTPDRA-----------SdadVSIWIELRSALeAAPeIKWQRTESGSIVTAa 247 
Q2HCC9             200 gASEYRTFVP-------VTVDNEGnESrqs--pagEtaeVSVWVHLRTALqAEPsITWYRSDSGTIITTd 260  Chaetomium globosu...
jgi:MYCTH_2307835  200 gGSEYRAFVP-------VTVDEEGnESrqha-gagDaaeISIWIHLRTALqAEPsIAWRRSESGAIVTvd 261 
XP_001593651       204 -GQSHLSFTS-------IFISSDAtETrtristnkDdadVSIYISLKTTIeSNPqIKWYRNGNGGIVSEG 265 
EPE29374           202 -GQPHLQFAS-------IQINEDGtEErk-----sSdadVSIYIDLRAVMeKEPkIAWAKTESGNVVTVG 258 
EKD14451           209 -GQDLLQFAT-------IVVQEDGsEVra----pkSdadVSIHINLRQVMeAEPkIRWARTDAGTVVTAG 266 
XP_007853293       182 -GDELIHL-------------VRKvPEyegieafnPtvrILISIDVEKAM-HDGdIMFYKINDGSLVTDG 236 
EDR05686           254 -HAGLIRLSATS-----SVKSSRP-QRwynrvnavPirfVTIAVDIRQAF-ALG-IEFYRTRRGYIISPG 314 
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