» » »

wormbook

WormBook

The Online Review of C. elegans Biology

PriessJames

SeydouxGeraldine

2007

© 2007 Creative Commons Attribution License

Table of Contents

Book Information
Cell biology
- Basement membranes James M. Kramer Created: September 1, 2005
- Cell-cycle regulation Sander van den Heuvel Created: September 21, 2005
- Cell division Karen Oegema and Tony Hyman Created: January 19, 2006
- Cell fusion Benjamin Podbilewicz Created: January 6, 2006
- Epithelial junctions and attachments Michel Labouesse Created: January 13, 2006
- Intracellular trafficking Barth D. Grant and Miyuki Sato Created: January 21, 2006
- Sarcomere assembly in C. elegans muscle Donald G. Moerman and Benjamin D. Williams Created: January 16, 2006
- Sperm motility and MSP Harold Smith Created: February 1, 2006
- The cadherin superfamily Jonathan Pettitt Created: December 29, 2005
- The cuticle Antony P. Page and Iain L. Johnstone Created: May 3, 2007
- The sensory cilia of Caenorhabditis elegans Peter N. Inglis, Guangshuo Ou, Michel R. Leroux, and Jonathan M. Scholey Created: November 27, 2006
Developmental control mechanisms
- Asymmetric cell division and axis formation in the embryo Pierre Gönczy and Lesilee S. Rose Created: October 15, 2005
- Embryological variation during nematode development Einhard Schierenberg Created: January 2, 2006
- Epidermal morphogenesis Andrew D. Chisholm and Jeff Hardin Created: December 1, 2005
- Gastrulation in C. elegans Jeremy Nance, Jen-Yi Lee, and Bob Goldstein Created: September 26, 2005
- Notch signaling in the C. elegans embryo James R. Priess Created: June 25, 2005
- Programmed cell death Barbara Conradt and Ding Xue Created: October 6, 2005
- The C. elegans intestine James D. McGhee Created: May 3, 2007
- The C. elegans pharynx: a model for organogenesis Susan E. Mango Created: January 22, 2007
- Translational control of maternal RNAs Thomas C. Evans and Craig P. Hunter Created: November 10, 2005
Disease models and drug discovery
- C. elegans and volatile anesthetics P.G. Morgan, E.-B. Kayser, and M.M. Sedensky Created: May 3, 2007
- Obesity and the regulation of fat metabolism Kaveh Ashrafi Created: May 3, 2007
Nematode evolution and ecology
- Introduction to nematode evolution and ecology David H.A. Fitch Created: August 31, 2005
- A quick tour of nematode diversity and the backbone of nematode phylogeny Paul De Ley Created: January 25, 2006
- Ecology of Caenorhabditis species Karin Kiontke and Walter Sudhaus Created: January 9, 2006
- Evolution of development in nematodes related to C. elegans Ralf J. Sommer Created: December 14, 2005
- Interactions with microbial pathogens Creg Darby Created: September 6, 2005
- Natural variation and population genetics of Caenorhabditis elegans Antoine Barrière and Marie-Anne Félix Created: December 26, 2005
- Nematode genome evolution Avril Coghlan Created: September 7, 2005
- The phylogenetic relationships of Caenorhabditis and other rhabditids Karin Kiontke and David H. A. Fitch Created: August 11, 2005
Genetics and genomics
- Introduction to genetics and genomics Jonathan Hodgkin Created: September 6, 2005
- Complementation Karen Yook Created: October 6, 2005
  - 1. A simple test for assigning a mutation to a genetic locus
  - 2. When the complementation test is not so simple
  - 3. Conclusion
  - 4. Acknowledgements
  - 5. References
- Essential genes Kenneth Kemphues Created: December 24, 2005
  - 1. Overview
  - 2. Types of essential genes
  - 3. Methods for identifying essential genes
  - 4. Redundancy of essential genes
  - 5. Pleiotropic essential gene phenotypes
  - 6. Summary
  - 7. References
- Gene duplications and genetic redundancy in C. elegans Alison Woollard Created: June 25, 2005
  - 1. Gene duplications and genetic redundancy
  - 2. Acknowledgements
  - 3. References
- Genetic enhancers Robert K. Herman and John Yochem Created: September 16, 2005
  - 1. Overview
  - 2. Dominant enhancers
  - 3. Genetic redundancy
  - 4. Enhancement of mutations in homologous genes
  - 5. Enhancement of mutations in functionally redundant but nonhomologous genes
  - 6. Recessive enhancers of non-null mutations
  - 7. References
- Genetic mosaics John Yochem and Robert K. Herman Created: December 27, 2005
  - 1. Overview
  - 2. How worm genetic mosaics are generated
  - 3. A few examples of mosaic analysis in worms
  - 4. Potential problems
  - 5. The continued importance of mosaic analysis
  - 6. References
- Genetic suppression Jonathan Hodgkin Created: December 27, 2005
  - 1. Introduction
  - 2. Intragenic suppression by same site replacement
  - 3. Intragenic suppression by compensatory second site mutation
  - 4. Intragenic suppression by altered splicing
  - 5. Intragenic suppression of dominant mutations by loss-of-function in cis
  - 6. Extragenic suppression — overview
  - 7. Informational suppression — overview
  - 8. Nonsense suppression
  - 9. Extragenic suppression by modified splicing
  - 10. Suppression by loss of NMD (Nonsense Mediated Decay)
  - 11. Extragenic suppression by non-specific physiological effects
  - 12. Suppression by altered gene dosage
  - 13. Bypass suppression
  - 14. Extragenic suppression by interacting components
  - 15. Suppression by removal of a toxic protein or protein complex
  - 16. Suppression by modulators of activity
  - 17. Suppression by epistasis
  - 18. References
- Karyotype, ploidy, and gene dosage Jonathan Hodgkin Created: June 25, 2005
  - 1. Normal karyotype
  - 2. Overview of chromosome organization
  - 3. Abnormal karyotype
  - 4. Tetraploids, triploids and haploids
  - 5. Aneuploids
  - 6. Chromosomal duplications
  - 7. Chromosomal deficiencies
  - 8. Dosage sensitive genes
  - 9. References
- C. elegans network biology: a beginning Fabio Piano, Kristin C. Gunsalus, David E. Hill, and Marc Vidal Created: August 21, 2006
  - 1. Introduction
  - 2. Network components: transcriptome and ORFeome
  - 3. Network connections: interactome
  - 4. Network perturbations: phenome
  - 5. Network dynamics: from transcriptome to localizome
  - 6. Network modeling: "omic" data integration
  - 7. Perspectives
  - 8. Acknowledgments
  - 9. References
- C. elegans noncoding RNA genes Shawn L. Stricklin, Sam Griffiths-Jones, and Sean R. Eddy Created: June 25, 2005
  - 1. Introduction
  - 2. Ribosomal RNAs
  - 3. Transfer RNAs
  - 4. Spliced leader RNAs
  - 5. Small nucleolar RNAs (snoRNAs)
  - 6. microRNAs (miRNAs)
  - 7. Other known RNAs
  - 8. ncRNA conservation
  - 9. Prospects for novel ncRNAs
  - 10. Acknowledgments
  - 11. References
- Genomic classification of protein-coding gene families Erich M. Schwarz Created: September 23, 2005
  - 1. Introduction
  - 2. Similarity, homology, and shared functions
  - 3. Classifying proteins
  - 4. Sorting proteins into homologs, orthologs and paralogs
  - 5. Protein classes in C. elegans
  - 6. Evolutionary history
  - 7. Functional classification
  - 8. Acknowledgements
  - 9. References
- Mitochondrial genetics Bernard Lemire Created: September 14, 2005
  - 1. Introduction
  - 2. Mitochondrial DNA structure
  - 3. mtDNA-encoded proteins
  - 4. Mitochondrial DNA inheritance
  - 5. Mitochondrial dynamics
  - 6.mtDNA copy number
  - 7. Role of mtDNA in development
  - 8. mtDNA mutations
  - 9. mtDNA and aging
  - 10. Perspectives
  - 11. Acknowledgements
  - 12. References
- Overview of gene structure John Spieth and Daniel Lawson Created: January 18, 2006
  - 1. What is a gene?
  - 2. Protein-coding genes
  - 3. Pseudogenes
  - 4. non-coding RNA genes
  - 5. Genomic organization
  - 6. Acknowledgements
  - 7. References
- Transposons in C. elegans Jean-Louis Bessereau Created: January 18, 2006
  - 1. C. elegans transposons, the catalog
  - 2. Mechanisms of Tc1 and Tc3 transposition
  - 3. Transposon insertion target
  - 4. Repair of the excision site
  - 5. Regulation of transposition
  - 6. Transposon-based tools for C. elegans research
  - 7. Acknowledgments
  - 8. References
- Genomics and biology of the nematode Caenorhabditis briggsae Bhagwati P. Gupta, Robert Johnsen, and Nansheng Chen Created: May 3, 2007
  - 1. Introduction
  - 2. Overview of the C. briggsae genome
  - 3. Evolutionary studies
  - 4. Genetic approach to study functions of C. briggsae genes
  - 5. Tools for genetic studies
  - 6. Acknowledgements
  - 7. Bibliography
- Oscheius tipulae Marie-Anne Félix Created: August 16, 2006
  - 1. Phylogenetic relationships
  - 2. Natural populations
  - 3. Basic biology in the laboratory
  - 4. Genetics
  - 5. Genome and molecular biology resources
  - 6. Developmental biology
  - 7. Acknowledgement
  - 8. References
- Pristionchus pacificus Ralf J. Sommer Created: August 14, 2006
  - 1. Biology
  - 2. Developmental biology
  - 3. Phylogeny
  - 4. Ecology
  - 5. Genetics
  - 6. Genomics
  - 7. Acknowledgements
  - 8. References
- Strongyloides spp. Mark E. Viney and James B. Lok Created: May 3, 2007
  - 1. Strongyloides is a genus of parasitic nematodes
  - 2. Strongyloides infection of humans
  - 3. Strongyloides in the wild
  - 4. Phylogeny, morphology and taxonomy
  - 5. The life-cycle
  - 6. Sex determination and genetics of the life-cycle
  - 7. Controlling the life-cycle
  - 8. Maintaining the life-cycle
  - 9. The parasitic phase of the life-cycle
  - 10. Genomics
  - 11. Acknowledgments
  - 12. References
- The biology and genome of Heterorhabditis bacteriophora Todd Ciche Created: May 3, 2007
  - 1. Introduction
  - 2. Discovery and diversity
  - 3. Life-cycle
  - 4. Parasitism
  - 5. Symbiosis
  - 6. Other biology of interest for Heterorhabditis
  - 7. Genetics and molecular biology of H. bacteriophora
  - 8. Status and future of the H. bacteriophora genome project
  - 9. Conclusion
  - 10. Acknowledgements
  - 11. References
- Biology and genome of Trichinella spiralis Makedonka Mitreva and Douglas P. Jasmer Created: November 23, 2006
  - 1. Introduction
  - 2. Molecular and cellular interactions with the host
  - 3. Use of molecular biology in Trichinella
  - 4. Previous genomic applications to study Trichinella
  - 5. Current genome sequencing project for Trichinella spiralis
  - 6. Acknowledgements
  - 7. References
Molecular biology
- Alternative splicing in C. elegans Alan M. Zahler Created: September 26, 2005
- C. elegans microRNAs Monica C. Vella and Frank J. Slack Created: September 21, 2005
- DNA repair Nigel O'Neil and Ann Rose Created: January 13, 2006
- Mechanism and regulation of translation in C. elegans Robert E. Rhoads, Tzvetanka D. Dinkova, and Nadejda L. Korneeva Created: January 28, 2006
- RNA-binding proteins Min-Ho Lee and Tim Schedl Created: April 18, 2006
- Roles of chromatin factors in C. elegans development Mingxue Cui and Min Han Created: May 3, 2007
- Trans-splicing and operons Thomas Blumenthal Created: June 25, 2005
- Transcription mechanisms T. Keith Blackwell and Amy K. Walker Created: September 5, 2006
- Transcriptional regulation Peter G. Okkema and Michael Krause Created: December 23, 2005
- Ubiquitin-mediated pathways in C.elegans Edward T. Kipreos Created: December 1, 2005
Neurobiology and behavior
- Egg-laying William R. Schafer Created: December 14, 2005
- Male mating behavior Maureen M. Barr and L. Rene Garcia Created: June 19, 2006
- Specification of the nervous system Oliver Hobert Created: August 8, 2005
- Synaptogenesis Yishi Jin Created: December 23, 2005
- Potassium channels in C. elegans L. Salkoff, A.D. Wei, B. Baban, A. Butler, G. Fawcett, G. Ferreira, and C.M. Santi Created: December 30, 2005
- Synaptic function Janet Richmond Created: December 30, 2005
- Acetylcholine James B. Rand Created: January 30, 2007
- Biogenic amine neurotransmitters in C. elegans Daniel L. Chase and Michael R. Koelle Created: February 20, 2007
- GABA Erik M. Jorgensen Created: August 31, 2005
- Ionotropic glutamate receptors: genetics, behavior and electrophysiology Penelope J. Brockie and Andres V. Maricq Created: January 19, 2006
- Chemosensation in C. elegans Cornelia I. Bargmann Created: October 25, 2006
- Mechanosensation Miriam B. Goodman Created: January 6, 2006
Post-embryonic development
- Gene expression changes associated with aging in C. elegans Tamara R. Golden and Simon Melov Created: February 12, 2007
Sex determination
Signal transduction
- Introduction to signal transduction Iva Greenwald Created: September 9, 2005
- Genomic overview of protein kinases Gerard Manning Created: December 13, 2005
- RTK/Ras/MAPK signaling Meera V. Sundaram Created: February 11, 2006
- Heterotrimeric G proteins in C. elegans Carol Bastiani and Jane Mendel Created: October 13, 2006
- Homologs of the Hh signalling network in C. elegans Thomas R. Bürglin and Patricia E. Kuwabara Created: January 28, 2006
- LIN-12/Notch signaling in C. elegans Iva Greenwald Created: August 8, 2005
- Nuclear hormone receptors in C. elegans Adam Antebi Created: January 3, 2006
- Signaling in the immune response Jonathan J. Ewbank Created: January 23, 2006
- Small GTPases Erik A. Lundquist Created: January 17, 2006
- TGF-β signaling Cathy Savage-Dunn Created: September 9, 2005
- The putative chemoreceptor families of C. elegans Hugh M. Robertson and James H. Thomas Created: January 6, 2006
- Wnt signaling David M. Eisenmann Created: June 25, 2005
The germ line
- Introduction to the germ line E. Jane Albert Hubbard and David Greenstein Created: September 1, 2005
- Control of oocyte meiotic maturation and fertilization David Greenstein Created: December 28, 2005
- Germline chromatin Christine E. Schaner and William G. Kelly Created: January 24, 2006
- Germline genomics Valerie Reinke Created: January 20, 2006
- Germline proliferation and its control Judith Kimble and Sarah L. Crittenden Created: August 15, 2005
- Specification of the germ line Susan Strome Created: July 28, 2005
- Spermatogenesis S.W. L'Hernault Created: February 20, 2006
WormMethods
- Behavior Anne C. Hart, ed Created: July 3, 2006
  - 1. Introduction
  - 2. Mechanosensation
  - 3. Osmotic avoidance
  - 4. Chemosensation
  - 5. Learning, adaptation and habituation
  - 6. Thermal responses
  - 7. Locomotion
  - 8. Feeding
  - 9. Egg-laying, males and mating
  - 10. Assays of C. elegans reproductive behaviors
  - 11. Defecation
  - 12. Galvanotaxis
  - 13. Circadian rhythms
  - 14. References
- Biochemistry and molecular biology Albertha J. M. Walhout and Simon J. Boulton Created: February 13, 2006
  - 1. The yeast two-hybrid system
  - 2. Tandem immuno-affinity purification
  - 3. Acknowledgments
  - 4. References
- Introduction and basics David Fay Created: February 17, 2006
  - 1. Fundamentals
  - 2. A word about nomenclature
  - 3. Types of mutations
  - 4. Genetic shorthand
  - 5. Feeding, growing, and maintaining worms
  - 6. Worm chromosomes
  - 7. Setting up crosses
  - 8. The big picture
  - 9. References
- Two-point mapping with genetic markers David Fay Created: June 14, 2006
  - 1. The basics
  - 2. Calculating map distances
  - 3. Other considerations
  - 4. References
- Three-point mapping with genetic markers David Fay Created: February 17, 2006
  - 1. Basics
  - 2. Saving recombinants
  - 3. Some words of caution
  - 4. Finding and picking recombinants
  - 5. Exit strategies
- Dominant mutations David Fay and Andy Spencer Created: February 17, 2006
  - 1. Basics
  - 2. Isolating dominant alleles
  - 3. Mapping a dominant mutation
  - 4. Different types of dominant mutations
  - 5. Genetic tests for dominance classes
  - 6. References
- Making compound mutants David Fay Created: June 14, 2006
  - 1. Background
  - 2. Making double mutants
  - 3. Using the power of counter-selection
  - 4. Crossing mutants into reporter lines
- Mapping with deficiencies and duplications David Fay Created: June 14, 2006
  - 1. Basics
  - 2. Setting up crosses with deficiencies
  - 3. Mapping with duplications
  - 4. References
- SNPs: Introduction and two-point mapping David Fay and Aaron Bender Created: February 17, 2006
  - 1. Basics
  - 2. The SNP database
  - 3. Using different types of SNPs
  - 4. Two-point mapping with SNPs
  - 5. Worm PCR protocol
  - 6. References
- SNPs: Three-point mapping David Fay Created: February 17, 2006
  - 1. The basics
  - 2. Examples of SNP mapping
  - 3. General SNP mapping strategies
- Suppressor mutations David Fay and Wade Johnson Created: February 17, 2006
  - 1. Basics
  - 2. Considerations and caveats
  - 3. Suppressor identification strategies
  - 4. Mapping suppressor mutations
- Synthetic and enhancer mutations David Fay Created: February 17, 2006
  - 1. Basics
  - 2. Making mapping strains
  - 3. Two-point mapping with genetic markers
  - 4. Three-point mapping with genetic markers
  - 5. Deficiency mapping
  - 6. Using SNPs to map synthetic mutants
- Genetic balancers Mark L. Edgley, David L. Baillie, Donald L. Riddle, and Ann M. Rose Created: April 6, 2006
  - 1. Introduction
  - 2. Types of balancers
  - 3. Practical considerations of balancer use
  - 4. The balancer map
  - 5. A field guide to balancers
  - 6. References
- Genetic dissection of developmental pathways Linda S. Huang and Paul W. Sternberg Created: June 14, 2006
  - 1. Introduction
  - 2. Epistasis analysis
  - 3. Epistasis analysis of switch regulation pathways
  - 4. Extragenic suppressors
  - 5. Prospects: use of new technologies for pathway analysis
  - 6. Conclusion
  - 7. Acknowledgements
  - 8. References
- Maintenance of C. elegans Theresa Stiernagle Created: February 11, 2006
  - 1. Introduction
  - 2. Acquiring strains from the Caenorhabditis Genetics Center (CGC)
  - 3. Preparation of growth media
  - 4. Culturing C. elegans on petri plates
  - 5. Growth of C. elegans in liquid medium
  - 6. Cleaning contaminated C. elegans stocks
  - 7. Freezing and recovery of C. elegans stocks
  - 8. Acknowledgements
  - 9. References
- Nomarski images for learning the anatomy, with tips for mosaic analysis John Yochem Created: January 24, 2006
  - 1. An overview
  - 2. The tutorial
  - 3. Acknowledgments
  - 4. References
- Profiling C. elegans gene expression with DNA microarrays Douglas S. Portman Created: January 20, 2006
  - 1. Planning your experiment
  - 2. Protocols
  - 3. Following up
  - 4. References
- Immunohistochemistry Janet S. Duerr Created: June 19, 2006
  - 1. Introduction
  - 2. Protocols and procedures
  - 3. References
- Protocols for large scale in situ hybridization on C. elegans larvae Tomoko Motohashi, Hiroaki Tabara, and Yuji Kohara Created: July 24, 2006
  - 1. Preparation of staged worms
  - 2. Fixation
  - 3. Hybridization and detection
  - 4. Reagents
- Reporter gene fusions Thomas Boulin, John F. Etchberger, and Oliver Hobert Created: April 5, 2006
  - 1. General considerations
  - 2. Protocols
  - 3. References
- RNA in situ hybridization of dissected gonads Min-Ho Lee and Tim Schedl Created: June 14, 2006
  - 1. Introduction
  - 2. General expression pattern
  - 3. Materials
  - 4. Procedure
  - 5. Acknowledgements
  - 6. References
- Methods in cell biology Shai Shaham, ed Created: January 2, 2006
  - 1. Introduction
  - 2. Visualizing cells and their components
  - 3. Protein-protein and protein-DNA interactions
  - 4. Specific methods in C. elegans cell biology
  - 5. Embryonic cell culture
  - 6. References
- Isolation of C. elegans and related nematodes Antoine Barrière and Marie-Anne Félix Created: July 17, 2006
  - 1. Introduction
  - 2. Isolation on an agar culture plate
  - 3. Baermann funnel
  - 4. Centrifugal flotation
  - 5. Identifying Caenorhabditis elegans and other species
  - 6. Typical time course and organization of an isolation experiment
  - 7. Culture conditions
  - 8. Freezing conditions
  - 9. References
- Pristionchus pacificus genetic protocols Andre Pires da Silva Created: July 17, 2006
  - 1. Introduction
  - 2. Protocols
  - 3. References
- Pristionchus pacificus genomics: from genetics to genome sequence Christoph Dieterich, Waltraud Roeseler, and Jagan Srinivasan Created: August 14, 2006
  - 1. Introduction
  - 2. Genomic resources for P. pacificus
  - 3. Genetic linkage map of P. pacificus
  - 4. Physical map of P. pacificus
  - 5. Bioinformatic resources for P. pacificus
  - 6. Acknowledgements
  - 7. References
- Strongyloides stercoralis: a model for translational research on parasitic nematode biology James B. Lok Created: February 17, 2007
  - 1. Introduction
  - 2. Laboratory hosts for S. stercoralis
  - 3. Biosafety
  - 4. In vitro cultivation of free-living stages
  - 5. Transgenesis
  - 6. Neuronal ablation
  - 7. Future prospects
  - 8. Afterword
  - 9. Acknowledgments
  - 10. References
- Neurophysiological methods in C. elegans: an introduction William R. Schafer Created: June 2, 2006
  - 1. Electrophysiology
  - 2. Optical imaging
  - 3. In vitro physiology
  - 4. References
- Caenorhabditis briggsae methods Scott E. Baird and Helen M. Chamberlin Created: December 18, 2006
  - 1. The Caenorhabditis briggsae model system
  - 2. Natural history and population biology
  - 3. Genetics
  - 4. Reverse genetic methods
  - 5. Online resources for C. briggsae genetics and genomics
  - 6. References
- Culture of embryonic C. elegans cells for electrophysiological and pharmacological analyses Laura Bianchi and Monica Driscoll Created: September 30, 2006
  - 1. Introduction
  - 2. A long-sought method for isolation and culture of embryonic C. elegans cells
  - 3. The protocol
  - 4. Cultured cells differentiate and behave in vitro as they do in vivo
  - 5. Electrophysiological techniques applicable to C. elegans cultured cells
  - 6. Acknowledgments
  - 7. References
- Electrophysiological recordings from the neuromuscular junction of C. elegans Janet E. Richmond Created: October 6, 2006
  - 1. Introduction
  - 2. Equipment
  - 3. Materials
  - 4. Dissection
  - 5. Recording
  - 6. References
- Electrophysiological recordings from the pharynx Alan Cook, Christopher J. Franks, and Lindy Holden-Dye Created: May 17, 2006
  - 1. Summary of available methods
  - 2. Protocol 1. Extrapharyngeal recordings (EPGs)
  - 3. Protocol 2. Intracellular recordings from the terminal bulb
  - 4. Protocol 3. Patch clamp recordings from the pharynx
  - 5. Acknowledgements
  - 6. References
- Heterologous expression of C. elegans ion channels in Xenopus oocytes Laura Bianchi and Monica Driscoll Created: August 1, 2006
  - 1. Introduction
  - 2. Basic characteristics of a Xenopus oocyte
  - 3. cDNA cloning into the appropriate vectors
  - 4. In vitro RNA synthesis
  - 5. Oocyte procurement
  - 6. Oocyte microinjection
  - 7. Two-electrode-voltage clamp
  - 8. Detecting proteins at the oocyte surface by fluorescent methods
  - 9. Extracting proteins from Xenopus oocytes for immunoprecipitations and western blotting
  - 10. Limitations of using Xenopus oocytes for expression of exogenous proteins
  - 11. Concluding remarks
  - 13. References
- Imaging the activity of neurons and muscles Rex A. Kerr Created: June 2, 2006
  - 1. Introduction
  - 2. Background and theory
  - 3. Protocols
  - 4. References
- Reverse genetics Julie Ahringer, ed Created: April 6, 2006
  - 1. Introduction to reverse genetics in C. elegans
  - 2. Using RNAi to knockdown gene function
  - 3. RNAi by injection
  - 4. RNAi by soaking
  - 5. RNAi feeding on agar plates
  - 6. RNAi feeding in liquid culture (96 well format)
  - 7. Construction and screening of deletion mutant libraries to generate C. elegans gene knockouts
  - 8. References
- Transformation and microinjection Thomas C. Evans, ed Created: April 6, 2006
  - 1. Introduction
  - 2. General considerations for DNA transformation
  - 3. Microinjection
  - 4. DNA transformation: Creating repetitive extrachromosomal arrays by microinjection
  - 5. DNA transformation: Creating complex arrays by microinjection
  - 6. Integration of extrachromosomal arrays
  - 7. DNA transformation by gene bombardment
  - 8. Future prospects: Targeted gene replacement
  - 9. Acknowledgments
  - 10. References
- Web resources for C. elegans studies Raymond Lee Created: December 28, 2005
  - 1. Introduction
  - 2. Portal and knowledge environment
  - 3. Literature search
  - 4. Gene function
  - 5. Sequence analysis-retrieval
  - 6. Sequence analysis-search for homologous sequences
  - 7. Genetics
  - 8. Cell and anatomy
  - 9. Concluding remark

Copyright © 2007 Creative Commons Attribution License

Help ǀ Contact Bookshelf