-- Molecular Cell Biology -- NCBI Bookshelf

Book Information
Acknowledgments
About the Authors
Preface
Supplements
1 The Dynamic Cell
- 1.1 Evolution: At the Core of Molecular Change
- 1.2 The Molecules of Life
- 1.3 The Architecture of Cells
- 1.4 The Life Cycle of Cells
- 1.5 Cells into Tissues
- 1.6 Molecular Cell Biology: An Integrated View of Cells at Work
2 Chemical Foundations
- 2.1 Covalent Bonds
- 2.2 Noncovalent Bonds
- 2.3 Chemical Equilibrium
- 2.4 Biochemical Energetics
- 2.5 Activation Energy and Reaction Rate
- Testing Yourself on the Concepts
- MCAT/GRE-Style Questions
- References
3 Protein Structure and Function
- 3.1 Hierarchical Structure of Proteins
- 3.2 Folding, Modification, and Degradation of Proteins
- 3.3 Functional Design of Proteins
- 3.4 Membrane Proteins
- 3.5 Purifying, Detecting, and Characterizing Proteins
- PERSPECTIVES for the Future
- PERSPECTIVES in the Literature
- Testing Yourself on the Concepts
- MCAT/GRE-Style Questions
- References
4 Nucleic Acids, the Genetic Code, and the Synthesis of Macromolecules
- 4.1 Structure of Nucleic Acids
- 4.2 Synthesis of Biopolymers: Rules of Macromolecular Carpentry
- 4.3 Nucleic Acid Synthesis
- 4.4 The Three Roles of RNA in Protein Synthesis
- 4.5 Stepwise Formation of Proteins on Ribosomes
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5 Biomembranes and the Subcellular Organization of Eukaryotic Cells
- 5.1 Microscopy and Cell Architecture
- 5.2 Purification of Cells and Their Parts
- 5.3 Biomembranes: Structural Organization and Basic Functions
- 5.4 Organelles of the Eukaryotic Cell
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6 Manipulating Cells and Viruses in Culture
- 6.1 Growth of Microorganisms in Culture
- 6.2 Growth of Animal Cells in Culture
- 6.3 Viruses: Structure, Function, and Uses
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7 Recombinant DNA and Genomics
- 7.1 DNA Cloning with Plasmid Vectors
- 7.2 Constructing DNA Libraries with λ Phage and Other Cloning Vectors
- 7.3 Identifying, Analyzing, and Sequencing Cloned DNA
- 7.4 Bioinformatics
- 7.5 Analyzing Specific Nucleic Acids in Complex Mixtures
- 7.6 Producing High Levels of Proteins from Cloned cDNAs
- 7.7 Polymerase Chain Reaction: An Alternative to Cloning
- 7.8 DNA Microarrays: Analyzing Genome-Wide Expression
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8 Genetic Analysis in Cell Biology
- 8.1 Mutations: Types and Causes
- 8.2 Isolation and Analysis of Mutants
- 8.3 Genetic Mapping of Mutations
- 8.4 Molecular Cloning of Genes Defined by Mutations
- 8.5 Gene Replacement and Transgenic Animals
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9 Molecular Structure of Genes and Chromosomes
- 9.1 Molecular Definition of a Gene
- 9.2 Chromosomal Organization of Genes and Noncoding DNA
- 9.3 Mobile DNA
- 9.4 Functional Rearrangements in Chromosomal DNA
- 9.5 Organizing Cellular DNA into Chromosomes
- 9.6 Morphology and Functional Elements of Eukaryotic Chromosomes
- 9.7 Organelle DNAs
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10 Regulation of Transcription Initiation
- 10.1 Bacterial Gene Control: The Jacob-Monod Model
- 10.2 Bacterial Transcription Initiation
- 10.3 Eukaryotic Gene Control: Purposes and General Principles
- 10.4 Regulatory Sequences in Eukaryotic Protein-Coding Genes
- 10.5 Eukaryotic Transcription Activators and Repressors
- 10.6 RNA Polymerase II Transcription-Initiation Complex
- 10.7 Molecular Mechanisms of Eukaryotic Transcriptional Control
- 10.8 Other Transcription Systems
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11 RNA Processing, Nuclear Transport, and Post-Transcriptional Control
- 11.1 Transcription Termination
- 11.2 Processing of Eukaryotic mRNA
- 11.3 Regulation of mRNA Processing
- 11.4 Signal-Mediated Transport through Nuclear Pore Complexes
- 11.5 Other Mechanisms of Post-Transcriptional Control
- 11.6 Processing of rRNA and tRNA
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12 DNA Replication, Repair, and Recombination
- 12.1 General Features of Chromosomal Replication
- 12.2 The DNA Replication Machinery
- 12.3 The Role of Topoisomerases in DNA Replication
- 12.4 DNA Damage and Repair and Their Role in Carcinogenesis
- 12.5 Recombination between Homologous DNA Sites
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13 Regulation of the Eukaryotic Cell Cycle
- 13.1 Overview of the Cell Cycle and Its Control
- 13.2 Biochemical Studies with Oocytes, Eggs, and Early Embryos
- 13.3 Genetic Studies with S. pombe
- 13.4 Molecular Mechanisms for Regulating Mitotic Events
- 13.5 Genetic Studies with S. cerevisiae
- 13.6 Cell-Cycle Control in Mammalian Cells
- 13.7 Checkpoints in Cell-Cycle Regulation
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14 Gene Control in Development
- 14.1 Cell-Type Specification and Mating-Type Conversion in Yeast
- 14.2 Cell-Type Specification in Animals
- 14.3 Anteroposterior Specification during Embryogenesis
- 14.4 Specification of Floral-Organ Identity in Arabidopsis
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15 Transport across Cell Membranes
- 15.1 Diffusion of Small Molecules across Phospholipid Bilayers
- 15.2 Overview of Membrane Transport Proteins
- 15.3 Uniporter-Catalyzed Transport
- 15.4 Intracellular Ion Environment and Membrane Electric Potential
- 15.5 Active Transport by ATP-Powered Pumps
- 15.6 Cotransport by Symporters and Antiporters
- 15.7 Transport across Epithelia
- 15.8 Osmosis, Water Channels, and the Regulation of Cell Volume
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16 Cellular Energetics: Glycolysis, Aerobic Oxidation, and Photosynthesis
- 16.1 Oxidation of Glucose and Fatty Acids to CO₂
- 16.2 Electron Transport and Oxidative Phosphorylation
- 16.3 Photosynthetic Stages and Light-Absorbing Pigments
- 16.4 Molecular Analysis of Photosystems
- 16.5 CO₂ Metabolism during Photosynthesis
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17 Protein Sorting: Organelle Biogenesis and Protein Secretion
- 17.1 Synthesis and Targeting of Mitochondrial and Chloroplast Proteins
- 17.2 Synthesis and Targeting of Peroxisomal Proteins
- 17.3 Overview of the Secretory Pathway
- 17.4 Translocation of Secretory Proteins across the ER Membrane
- 17.5 Insertion of Membrane Proteins into the ER Membrane
- 17.6 Post-Translational Modifications and Quality Control in the Rough ER
- 17.7 Protein Glycosylation in the ER and Golgi Complex
- 17.8 Golgi and Post-Golgi Protein Sorting and Proteolytic Processing
- 17.9 Receptor-Mediated Endocytosis and the Sorting of Internalized Proteins
- 17.10 Molecular Mechanisms of Vesicular Traffic
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18 Cell Motility and Shape I: Microfilaments
- 18.1 The Actin Cytoskeleton
- 18.2 The Dynamics of Actin Assembly
- 18.3 Myosin: The Actin Motor Protein
- 18.4 Muscle: A Specialized Contractile Machine
- 18.5 Actin and Myosin in Nonmuscle Cells
- 18.6 Cell Locomotion
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19 Cell Motility and Shape II: Microtubules and Intermediate Filaments
- 19.1 Microtubule Structures
- 19.2 Microtubule Dynamics and Associated Proteins
- 19.3 Kinesin, Dynein, and Intracellular Transport
- 19.4 Cilia and Flagella: Structure and Movement
- 19.5 Microtubule Dynamics and Motor Proteins during Mitosis
- 19.6 Intermediate Filaments
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20 Cell-to-Cell Signaling: Hormones and Receptors
- 20.1 Overview of Extracellular Signaling
- 20.2 Identification and Purification of Cell-Surface Receptors
- 20.3 G Protein –Coupled Receptors and Their Effectors
- 20.4 Receptor Tyrosine Kinases and Ras
- 20.5 MAP Kinase Pathways
- 20.6 Second Messengers
- 20.7 Interaction and Regulation of Signaling Pathways
- 20.8 From Plasma Membrane to Nucleus
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21 Nerve Cells
- 21.1 Overview of Neuron Structure and Function
- 21.2 The Action Potential and Conduction of Electric Impulses
- 21.3 Molecular Properties of Voltage-Gated Ion Channels
- 21.4 Neurotransmitters, Synapses, and Impulse Transmission
- 21.5 Neurotransmitter Receptors
- 21.6 Sensory Transduction
- 21.7 Learning and Memory
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22 Integrating Cells into Tissues
- 22.1 Cell-Cell Adhesion and Communication
- 22.2 Cell-Matrix Adhesion
- 22.3 Collagen: The Fibrous Proteins of the Matrix
- 22.4 Noncollagen Components of the Extracellular Matrix
- 22.5 The Dynamic Plant Cell Wall
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23 Cell Interactions in Development
- 23.1 Dorsoventral Patterning by TGFβ-Superfamily Proteins
- 23.2 Tissue Patterning by Hedgehog and Wingless
- 23.3 Molecular Mechanisms of Responses to Morphogens
- 23.4 Reciprocal and Lateral Inductive Interactions
- 23.5 Overview of Neuronal Outgrowth
- 23.6 Directional Control of Neuronal Outgrowth
- 23.7 Formation of Topographic Maps and Synapses
- 23.8 Cell Death and Its Regulation
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24 Cancer
- 24.1 Tumor Cells and the Onset of Cancer
- 24.2 Proto-Oncogenes and Tumor-Suppressor Genes
- 24.3 Oncogenic Mutations Affecting Cell Proliferation
- 24.4 Mutations Causing Loss of Cell-Cycle Control
- 24.5 Mutations Affecting Genome Stability
- PERSPECTIVES for the Future
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