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Figure 7.3

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   ORF scans are complicated by introns

The nucleotide sequence of a short gene containing a single intron is shown. The correct amino acid sequence of the protein translated from the gene is given immediately below the nucleotide sequence: in this sequence the intron has been left out because it is removed from the transcript before the mRNA is translated into protein. In the lower line, the sequence has been translated without realizing that an intron is present. As a result of this error, the amino acid sequence appears to terminate within the intron. The amino acid sequences have been written using the one-letter abbreviations (see Table 3.1). The genetic code was described in Section 3.3.2; introns are covered in detail in Section 10.1.3.