Studying DNA -- Genomes 2 -- NCBI Bookshelf

» » » »

Table 4.4

Sizes of human genomic libraries prepared in different types of cloning vector

		Number of clones*
Type of vector	Insert size (kb)	P = 95%	P = 99%
λ replacement	18	532 500	820 000
Cosmid, fosmid	40	240 000	370 000
P1	125	77 000	118 000
BAC, PAC	300	32 000	50 000
YAC	600	16 000	24 500
Mega-YAC	1400	6850	10 500

Calculated from the equation:

where N is the number of clones required, P is the probability that any given segment of the genome is present in the library, a is the average size of the DNA fragments inserted into the vector, and b is the size of the genome.