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The Dynamic Synapse
Molecular Methods in Ionotropic Receptor Biology
Kittler
Josef T.
University College of London, UK
Moss
Stephen J.
University College of London, UK
CRC Press, Taylor & Francis Group
0-8493-1891-2
978-0-8493-1891-7
2006
© 2006 by
Taylor & Francis Group, LLC
Frontiers in Neuroscience
Table of Contents
Preface
The Editors
Contributors
1 Methods for Uncovering the Mechanisms of AMPA Receptor Trafficking
Sophie Restituito and Edward B. Ziff
INTRODUCTION
APPROACHES USED IN THE STUDY OF AMPA RECEPTOR TRAFFICKING
SYNTHESIS, SUMMARY AND SPECULATION
REFERENCES
2 Long-Term Plasticity at Inhibitory Synapses: A Phenomenon That Has Been Overlooked
Jean-Luc Gaiarsa and Yehezkel Ben-Ari
INTRODUCTION
LONG-TERM CHANGES IN THE STRENGTH OF INHIBITORY SYNAPSES
CONCLUSION
REFERENCES
3 New Tricks for an Old Dog: Proteomics of the PSD
Bryen A. Jordan, Brian D. Fernholz, Thomas A. Neubert, and Edward B. Ziff
INTRODUCTION
THE BRAIN AND THE PSD
METHODS
A COMPARISON OF PSD PROTEOMICS STUDIES
CHALLENGES
CONCLUSIONS
REFERENCES
4 Phosphorylation Site-Specific Antibodies as Research Tools in Studies of Native GABA
A
Receptors
Jasmina N. Jovanovic
INTRODUCTION
DESIGN, PRODUCTION AND CHARACTERIZATION OF GABA
A
RECEPTOR PHOSPHO-SITE SPECIFIC ANTIBODIES
CONCLUDING REMARKS
REFERENCES
5 Protein Palmitoylation by DHHC Protein Family
Yuko Fukata, David S. Bredt, and Masaki Fukata
INTRODUCTION
OVERVIEW OF PAT SCREENING
PROTOCOLS
REFERENCES
6 Studying the Localization, Surface Stability and Endocytosis of Neurotransmitter Receptors by Antibody Labeling and Biotinylation Approaches
I. Lorena Arancibia-Cárcamo, Benjamin P. Fairfax, Stephen J. Moss, and Josef T. Kittler
INTRODUCTION
ENDOCYTOSIS
METHODS FOR STUDYING ENDOCYTOSIS
CONCLUSION
REFERENCES
7 Visualization of AMPAR Trafficking and Surface Expression
Pavel V. Perestenko and Jeremy M. Henley
INTRODUCTION
FLUORESCENT PROTEIN TOOLS
APPLICATION OF XFP IMAGING TO AMPARS
FUTURE IMAGING POSSIBILITIES
CONCLUSION
REFERENCES
8 Neurotransmitter Dynamics
Sabine Lévi and Antoine Triller
INTRODUCTION
RECEPTOR TRAFFICKING
RECEPTOR DYNAMICS
REFERENCES
9 Receptor Dynamics at the Cell Surface Studied Using Functional Tagging
Philip Thomas and Trevor G. Smart
INTRODUCTION
PERSPECTIVE ON NONFUNCTIONAL RECEPTOR TAGGING TECHNIQUES
EARLY STUDIES USING FUNCTIONAL TAGGING
THE NATURE OF THE EPITOPE: CRITERIA FOR SELECTING A FUNCTIONAL TAG
ADVANTAGES OF THE FUNCTIONAL TAG IN THE STUDY OF MOBILE RECEPTORS
AMPA RECEPTOR RECTIFICATION AND SYNAPTIC “AMPAFICATION”
MK801 CHANNEL BLOCK EXPOSES THE TRANSIENT NATURE OF SYNAPTIC NMDA RECEPTORS
SYNAPTIC INHIBITION: THE MOBILITY OF EXTRASYNAPTIC GABA
A
RECEPTORS
PHOTORECEPTIVE POTASSIUM CHANNELS AS SWITCHES OF NEURONAL EXCITABILITY
DISADVANTAGES AND LIMITATIONS OF THE FUNCTIONAL TAG
CONCLUSION
REFERENCES
10 RNAi and Applications in Neurobiology
Tija C. Jacob
INTRODUCTION TO RNAi
RNAi MECHANISM
GENERATING RNAi — TYPES OF RNAi
RNAi DELIVERY — SPECIFIC METHODS FOR EMPLOYING RNAi IN VARIOUS EXPERIMENTAL SYSTEMS
RNAi EXPERIMENTAL DESIGN STRATEGY
CONCLUSION
REFERENCES
11 Transfecting and Transducing Neurons with Synthetic Nucleic Acids and Biologically Active Macromolecules
Josef T. Kittler, Jonathan G. Hanley, and John T. R. Isaac
INTRODUCTION
METHODS FOR NEURONAL GENE TRANSFER
VIRAL APPROACHES
CHEMICAL AND PHYSICAL TRANSFECTION APPROACHES
ELECTRICAL APPROACHES: ELECTROPORATION AND NUCLEOFECTION
TRANSDUCING NEURONS WITH BIOLOGICALLY ACTIVE PEPTIDES, PROTEINS AND ANTIBODIES
FUNCTIONAL STUDIES USING FUNCTION BLOCKING AND PROTEIN–PROTEIN INTERACTION-BLOCKING PEPTIDES, PROTEINS AND ANTIBODIES
TRANSDUCING NEURONS WITH MEMBRANE-PERMEANT PEPTIDES AND PROTEINS
CONCLUSION
REFERENCES
12 Acute
In Vivo
Expression of Recombinant Proteins in Rat Brain Using Sindbis Virus
Hélène Marie and Robert C. Malenka
INTRODUCTION
GENERATION OF SINDBIS VIRAL PARTICLES
IN VIVO
INJECTION OF VIRAL PARTICLES
USE OF ACUTE
IN VIVO
EXPRESSION OF RECOMBINANT PROTEINS FOR CELL-BASED ASSAYS
CONCLUSIONS
REFERENCES
13 Lentivirus-Based Genetic Manipulations in Neurons
In Vivo
Pavel Osten, Tanjew Dittgen, and Pawel Licznerski
INTRODUCTION
LENTIVIRUS-BASED VECTOR SYSTEMS
LENTIVIRUS-BASED HETEROLOGOUS EXPRESSION
LENTIVIRUS-BASED GENETIC MANIPULATIONS
DELIVERY OF LENTIVIRAL PARTICLES INTO THE BRAIN
EXPERIMENTAL APPLICATIONS
CONCLUSIONS
REFERENCES
14 AMPA Receptor Phosphorylation in Synaptic Plasticity: Insights from Knockin Mice
Hey-Kyoung Lee
INTRODUCTION
AMPA RECEPTORS AND CHANGES IN PHOSPHORYLATION DURING LTP AND LTD
CHANGES IN GLUR1 WITH LTP AND LTD
GLUR1 PHOSPHORYLATION: EARLY EXPRESSION VERSUS LATE MAINTENANCE OF LTP?
DIFFERENT MECHANISMS OF LTP IN YOUNG VERSUS OLD
ROLE OF PKA IN LTP
GLUR1 PHOSPHORYLATION SITES IN LTD AND RECEPTOR TRAFFICKING
POTENTIAL INTERACTION BETWEEN GLUR1 AND GLUR2 PHOSPHORYLATION SITES DURING LTD
CONCLUSION
REFERENCES
15 Genomic and Post-Genomic Tools for Studying Synapse Biology
Josef T. Kittler and Peter L. Oliver
INTRODUCTION
ORFEOME PROJECTS: THE MAMMALIAN GENE COLLECTION AND OTHER PROVIDERS OF FULL-LENGTH cDNA COLLECTIONS
INFERRING NEW GENE FUNCTION FROM PROTEIN-PROTEIN INTERACTION DATASETS
WHOLE GENOME shRNAi LIBRARIES
THE MOUSE AS A CORNERSTONE FOR INTEGRATING GENOMIC AND POSTGENOMIC RESOURCES
CONCLUSION
REFERENCES
List of Figures
Copyright © 2006 by
Taylor & Francis Group, LLC
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