| Organisms | bacteria and cyanobacteria | protists, fungi, plants, and animals |
| Cell size | generally 1 to 10 μm in linear dimension | generally 5 to 100 μm in linear dimension |
| Metabolism | anaerobic or aerobic | aerobic |
| Organelles | few or none | nucleus, mitochondria, chloroplasts, endoplasmic reticulum, etc. |
| DNA | circular DNA in cytoplasm | very long linear DNA molecules containing many noncoding regions; bounded by nuclear envelope |
| RNA and protein | RNA and protein synthesized in same compartment | RNA synthesized and processed in nucleus; proteins synthesized in cytoplasm |
| Cytoplasm | no cytoskeleton: cytoplasmic streaming, endocytosis, and exocytosis all absent | cytoskeleton composed of protein filaments; cytoplasmic streaming; endocytosis and exocytosis |
| Cell division | chromosomes pulled apart by attachments to plasma membrane | chromosomes pulled apart by cytoskeletal spindle apparatus |
| Cellular organization | mainly unicellular | mainly multicellular, with differentiation of many types |