Figure 8-65. The antisense RNA strategy for generating dominant negative mutations.

Figure 8-65The antisense RNA strategy for generating dominant negative mutations

Mutant genes that have been engineered to produce antisense RNA, which is complementary in sequence to the RNA made by the normal gene X, can cause double-stranded RNA to form inside cells. If a large excess of the antisense RNA is produced, it can hybridize with—and thereby inactivate—most of the normal RNA produced by gene X. Although in the future it may become possible to inactivate any gene in this way, at present the technique seems to work for some genes but not others.

From: Studying Gene Expression and Function

Cover of Molecular Biology of the Cell
Molecular Biology of the Cell. 4th edition.
Alberts B, Johnson A, Lewis J, et al.
New York: Garland Science; 2002.
Copyright © 2002, Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter; Copyright © 1983, 1989, 1994, Bruce Alberts, Dennis Bray, Julian Lewis, Martin Raff, Keith Roberts, and James D. Watson .

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