CID/ML#Target NameIC50 (nM) [SID, AID]Anti-target Name(s)IC50*(μM) [SID, AID]Fold SelectiveSecondary Assay(s) Name: IC50 (nM) [SID, AID]
CID 24856225/ML174PME-110 [SID 99206500, AID 463124]> 30 serine hydrolases, including APEH, PREP, CES, FAS, ABHD10> 1 [SID 99206500, AID 463149]>100LC-MS/MS assay: [SID 99206500, AID 463090]
Cytox assay: [SID 99206500, AID 463091]
Gel-based ABPP IC50: 10 nM [SID 99206500, AID 463124]
Cell-based assay: [SID 99206500, AID 463131]
Endogenous enzyme assay: [SID 99206500, AID 463149; SID 92709579, AID 463146]
Demethylation assay: [SID 99206500, AID 463132]
*

IC50 of the anti-target is defined as greater than the test compound concentration at which less than or equal to 50% inhibition of the anti-target is observed, which is reported in AID 463149. For SID 99206500, no anti-targets were observed at 1 μM in AID 463149, so the IC50 is reported as > 1 μM.

Fold-selectivity was calculated as: > IC50 for anti-target/IC50 for target

IC50 of the anti-target is defined as greater than the test compound concentration at which less than or equal to 50% inhibition of the anti-target is observed, which is reported in AID 463149. For SID 99206500, no anti-targets were observed at 1 μM in AID 463149, so the IC50 is reported as > 1 μM.

Fold-selectivity was calculated as: > IC50 for anti-target/IC50 for target

From: Probe Development Efforts to Identify Novel Inhibitors of Protein Phosphatase Methylesterase-1 (PME-1)

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Probe Reports from the NIH Molecular Libraries Program [Internet].

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