Figure 4. Isotope-coded affinity tags (ICAT).

Figure 4Isotope-coded affinity tags (ICAT)

A class of reagents called “isotope coded affinity tags” (ICAT reagents) are used to perform quantitative proteomic analyses based upon automated tandem mass spectrometry, stable isotope dilution theory, and a suite of bioinformatic tools for data analysis. Stable isotope signatures are introduced into proteins at specific sites via chemical reactions. These signatures are later deconvoluted by a mass spectrometer and serve as the basis for accurate quantification of each labeled protein. The objective of the initial implementation of this technology has been quantitative protein profiling. The method is schematically illustrated in Figure 5. SOURCE: Courtesy of Ruedi Aebersold, Institute for Systems Biology, Seattle, Washington.


Cover of Defining the Mandate of Proteomics in the Post-Genomics Era
Defining the Mandate of Proteomics in the Post-Genomics Era: Workshop Report.
National Research Council (US).
Washington (DC): National Academies Press (US); 2002.
Copyright © 2002, National Academy of Sciences.

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