TABLE 6.1

Peptides Derived from the Mouse TRPM4 Sequence Used as Antigens to Immunize Rabbits

PeptideAmino acid ResiduesNumber of Identical Amino Acids with the Sequence ofSpecific/Sensitive in Western Blot for TRPM4 in
  _____________________________________________________________________
  mTRPM4mTRPM5COS CellsPrimary Tissue
5781919YesYes
6791717(Yes)No
732181810NoNo
733991NoNo
73418187NoNo
73514146NoNo

Note: For the relative position of the sequences within the TRPM4 primary sequence, see Figure 6.1. The TRPM4 and TRPM5 amino acid sequences are closely related, but TRPM5 was not detectable by any of the sera.

From: Chapter 6, Lessons of Studying TRP Channels with Antibodies

Cover of TRP Channels
TRP Channels.
Zhu MX, editor.
Boca Raton (FL): CRC Press; 2011.
Copyright © 2011 by Taylor and Francis Group, LLC.

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