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FIGURE 3-1. Detection of antibodies against recombinant nucleocapsid protein of SCoV in animal sera by Western blot assay.

FIGURE 3-1Detection of antibodies against recombinant nucleocapsid protein of SCoV in animal sera by Western blot assay

Recombinant nuleocapsid protein (NP, 49.6 kD) was used as an antigen to detect anti-ScoV antibodies in animal sera. Protein A-HRP was used as a secondary antibody, and reactive bands were visualized by the enhanced chemiluminesence Western blotting system. A serum sample from a convalescent SARS patient was used as a positive control. Blots reacted with animal (SZ2, SZ3, SZ11, SZ17, SZ7, SZ16, or SZ19) or human sera are indicated. Results from the neutralization test for ScoV-specific antibodies in these serum samples are also shown.

From: ISOLATION AND CHARACTERIZATION OF VIRUSES RELATED TO THE SARS CORONAVIRUS FROM ANIMALS IN SOUTHERN CHINA

Cover of Learning from SARS
Learning from SARS: Preparing for the Next Disease Outbreak: Workshop Summary.
Institute of Medicine (US) Forum on Microbial Threats; Knobler S, Mahmoud A, Lemon S, et al., editors.
Washington (DC): National Academies Press (US); 2004.
Copyright 2003 AAAS.

Reprinted with permission from Guan et al., 2003

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