Table 1. Partial (informal) view of BAO annotations and relationships to describe assays related to the screening campaigns, “Identification of inhibitors of Kruppel-like factor 5” and “Novel allosteric modulators of the M5 muscarinic receptor.”

Screen. campaign"Identification of inhibitors of Kruppel-like factor 5"
AID170019731825
Assay StagePrimary AssayConfirmatory AssaySecondary Assay
Relationshiphas confirmatory assay 1973,
has counter assay 1825
has primary assay 1700has primary assay 1700
Assay Measurement Throughput QualitySingle concentration single measurementConcentration response multiple replicatesSingle concentration single measurement
Endpoint% InhibitionIC50% Inhibition
Assay FormatCell-based formatCell-based formatCell-based format
Assay DesignLuciferase inductionLuciferase inductionViability reporter, ATP content
Meta TargetRegulation of transcriptionRegulation of transcriptionCell death
Detection TechnologyLuminescenceLuminescenceLuminescence
Measured EntityLuciferaseLuciferaseATP
Screen. campaign"Novel allosteric modulators of the M5 muscarinic receptor"
AID266521942206
Assay StagePrimaryConfirmatorySecondary
Relationshiphas confirmatory assay 2194,
has counter assay 2206
has primary assay 2665has primary assay 2665
Assay Measurement Throughput QualitySingle concentration single measurementConcentration response multiple replicatesConcentration response multiple replicates
EndpointMaximal activationEC50EC50
Assay FormatCell-based formatBiochemical formatCell-based format
Assay DesignCalcium redistributionRadioligand bindingCalcium redistribution
Meta TargetMuscarinic acetylcholine receptor M5Muscarinic acetylcholine receptor M5Muscarinic acetylcholine receptor M1
Detection TechnologyFluorescence intensityScintillation counting, filter assayFluorescence intensity
Measured EntityCalcium(3H)-NMSCalcium

From: Development and Applications of the Bioassay Ontology (BAO) to Describe and Categorize High-Throughput Assays

Cover of Assay Guidance Manual
Assay Guidance Manual [Internet].
Sittampalam GS, Gal-Edd N, Arkin M, et al., editors.
Copyright Notice

All Assay Guidance Manual content, except where otherwise noted, is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported license (CC BY-NC-SA 3.0), which permits copying, distribution, transmission, and adaptation of the work, provided the original work is properly cited and not used for commercial purposes. Any altered, transformed, or adapted form of the work may only be distributed under the same or similar license to this one.

NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.