Figure 2 – Examples of Competitive Inhibition where Substrate (S) and Inhibitor (I) binding events are mutually exclusive

Figure 2 – Examples of Competitive Inhibition where Substrate (S) and Inhibitor (I) binding events are mutually exclusive. (a) Classical model for competitive inhibition where S and I compete for the same precise region of the active site. (b) I does not bind to the active site, but sterically hinders S binding. (c) S and I binding sites are overlapping. (d) S and I share a common binding pocket on the enzyme. (e) I binding can result in a conformational change that prevents S binding (and vice versa). This was adapted from Segal, Enzyme Kinetics.

From: Mechanism of Action assays for Enzymes

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Assay Guidance Manual [Internet].
Sittampalam GS, Gal-Edd N, Arkin M, et al., editors.
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