Figure 5: AlphaScreen and AlphaLisa

Figure 5: AlphaScreen and AlphaLisa. Left: Binding of biological partners (represented by small ovals A and B) brings Donor and Acceptor beads (represented by the large blue and yellow circles) into close proximity (≤200 nm) and thus a fluorescent signal between 520–620 nm is produced in the case of AlphaScreen and 615 nm in the case of the AlphaLisa. When there is no binding between biological partners, Donor and Acceptor beads are not in close proximity. Singlet oxygen decays and no signal are produced. Right: comparison of emission spectra for AlphaScreen (red) and AlphaLISA (blue).

From: Inhibition of Protein-Protein Interactions: Non-Cellular Assay Formats

Cover of Assay Guidance Manual
Assay Guidance Manual [Internet].
Sittampalam GS, Coussens NP, Nelson H, et al., editors.
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