Figure 2: . Diagram of a fluorescence polarization assay.

Figure 2:

Diagram of a fluorescence polarization assay. Rapidly rotating small molecule fluorophore gives low FP signal (low mP). The association of a relatively large molecule, such as a protein, with the small molecule fluorophore slows down the motion of the fluorophore, leading to increased FP signal.

From: Inhibition of Protein-Protein Interactions: Non-Cellular Assay Formats

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Assay Guidance Manual [Internet].
Sittampalam GS, Coussens NP, Nelson H, et al., editors.
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