Figure 9. . Scatter plots from two exemplar screens.

Figure 9.

Scatter plots from two exemplar screens. Plots A and B show plate to plate variability as assessed by controls (different colors for different controls). It can be seen that the signals (Y-axis) for each kind of control are similar from plate to plate when analyzing all plates (X-axis) indicating low plate-plate variation while in plot B there is dramatic change from plate to plate, which could be corrected by some appropriate normalization method if the variation is consistent for all kinds of controls, or one needs to consider dropping some plate/wells with inconsistent variation.

From: Cell-Based RNAi Assay Development for HTS

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Assay Guidance Manual [Internet].
Sittampalam GS, Coussens NP, Nelson H, et al., editors.
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