TABLE A6-1High-throughput technologies used to study adaptive immunity and the scale of information generated

High-throughput technologyApplicationsData ScaleRefs.
Single B cell-cloningAntibody discovery; repertoire analysis~102Tiller et al., 2008; Smith et al., 2009; Wrammert et al., 2008; Meijer et al., 2006; Poulsen et al., 2007; Wiberg et al., 2006
Microengraved devicesSingle cell functional analysis, antibody and TCR specificity; antibody discovery; T cell cytokine reactivity; transcriptional profiling~105Tokimitsu et al., 2007; Jin et al., 2009; Love et al., 2006; Ogunniyi et al., 2009; Story et al., 2008; Song et al., 2010; Han et al., 2010; Flatz et al., 2011; Kwong et al., 2009
Protein microarraysAntigen discovery from pathogens, cancer and autoimmune disease; serological antibody profiling; vaccine development~103–104Davies et al., 2005; Felgner et al., 2009; Kunnath-Velayudhan et al., 2010; Robinson et al., 2002; Gnjatic et al., 2009; Reddy et al., 2011; Legutki et al., 2010
Next generation DNA sequencingAntibody and TCR repertoire analysis, immunological biomarker discovery, clinical monitoring, antibody library quality control, monoclonal antibody discovery~106–107Weinstein et al., 2009; Jiang et al., 2011; Boyd et al., 2009; Freeman et al., 2009; Robins et al., 2009, 2010; Warren et al., forthcoming; Glanville et al., 2009; Ge et al., 2010; Ravn et al., 2010; Reddy et al., 2010

From: A6, SYSTEMS ANALYSIS OF ADAPTIVE IMMUNITY BY UTILIZATION OF HIGH-THROUGHPUT TECHNOLOGIES

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The Science and Applications of Synthetic and Systems Biology: Workshop Summary.
Institute of Medicine (US) Forum on Microbial Threats.
Washington (DC): National Academies Press (US); 2011.
Copyright © 2011, National Academy of Sciences.

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