Genetic signal converters
Genetic signal converters

FIGURE A12-2Genetic signal converters

(a) Analog-to-digital converter circuit that enables the discretization of analog inputs. The circuit is composed of a bank of toggle switches that have increasing response thresholds so that sequential toggling is achieved as input levels increase. The design could enable different natural or synthetic pathways to be activated depending on distinct input ranges, which may be useful in cell-based biosensing applications. Inputs into promoters and logic operations are shown explicitly except when the promoter (P) name is italicized, which represents an inducible promoter. (b) Digital-to-analog converter circuit that enables the programming of defined promoter activity based on combinatorial inputs. The circuit is composed of a bank of recombinase-based switches, known as single-invertase memory modules (SIMMs) (Friedland et al., 2009). Each SIMM is composed of an inverted promoter and a recombinase gene located between its cognate recognition sites, indicated by the arrows. Upon the combinatorial addition of inducers that activate specific Pwrite promoters, different SIMMs will be flipped, enabling promoters of varying strength to drive green fluorescent protein (GFP) expression. This allows combinatorial programming of different levels of promoter activity.


Cover of The Science and Applications of Synthetic and Systems Biology
The Science and Applications of Synthetic and Systems Biology: Workshop Summary.
Institute of Medicine (US) Forum on Microbial Threats.
Washington (DC): National Academies Press (US); 2011.
Copyright © 2011, National Academy of Sciences.

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