Figure 10.4. Use of the Genebridge 4 radiation hybrid panel for physical mapping.

Figure 10.4Use of the Genebridge 4 radiation hybrid panel for physical mapping

The panel consists of 93 hamster cells each containing on average 32% of the human genome in the form of many small fragments (average size 25 Mb). A central database stores data on the presence or absence in each hybrid of hundreds of markers from known chromosomal locations. A new STS is mapped by attempting to PCR-amplify it from each of the 93 hybrids, and comparing the pattern of positives and negatives with patterns in the database.

From: Chapter 10, Physical and transcript mapping

Cover of Human Molecular Genetics
Human Molecular Genetics. 2nd edition.
Strachan T, Read AP.
New York: Wiley-Liss; 1999.
Copyright © 1999, Garland Science.

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