Table 4.1Restriction endonucleases

EnzymeSourceSequence cutAverage expected fragment size (kb) in human DNA a
AluI Arthrobacter luteus AGCT0.3
HaeIII Hemophilus aegyptus GGCC0.6
TaqI Thermus aquaticus TCGA1.4
MnlI Moraxella nonliquefaciens CCTC/GAGG0.4
HindIII Hemophilus influenzae Rd AAGCTT3.1
EcoRIEscherichia coli R factorGAATTC3.1
BamHI Bacillus amyloliquefaciens H GGATCC7.0
PstI Providencia stuartii CTGCAG7.0
MstIMicrocoleus speciesCCTNAGG c 7.0
SmaI Serratia marcescens CCCGGG78
BssHII Bacillus stearothermophilus GCGCGC390 b
NotI Norcadia otitidis-caviarum GCGGCCGC9766 b

Assuming 40% G + C, and a CpG frequency 20% of that expected.


Observed average sizes are often lower than these estimates.


N = A, C, G or T.

Note: Names are normally derived from the first letter of the genus and the first two letters of the species name, e.g. PstI is the first restriction nuclease to have been isolated from Providencia stuartii. MnlI is an example of an enzyme whose recognition sequence is not palindromic. So-called rare-cutters often have recognition sequences containing one or more CpG dinucleotides and cut vertebrate DNA comparatively infrequently.

From: Chapter 4, Cell-based DNA cloning

Cover of Human Molecular Genetics
Human Molecular Genetics. 2nd edition.
Strachan T, Read AP.
New York: Wiley-Liss; 1999.
Copyright © 1999, Garland Science.

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