Figure 8. Representative examples of DNA image cytometry and fluorescence-in-situ hybridization (FISH) analyses on decoy cells.

Figure 8

Representative examples of DNA image cytometry and fluorescence-in-situ hybridization (FISH) analyses on decoy cells. Due to the viral DNA content, image cytometry invariably shows highly aneuploid patterns, which should not be mistaken as evidence for malignancy. FISH analyses, here with UroVysionTM probes to detect chromosomes 3, 7, 17 (chromosome enumeration probes - CEP), and 9p21 (single locus specific identifier- LSI) demonstrate regular copy numbers (n=2) and confirm the benign nature of decoy cells. The DNA probes are directly labeled with the four dyes SpectrumRed (CEP 3), SpectrumGreen (CEP 7), SpectrumAqua (CEP 17), and SpectrumGold (LSI 9p21).

From: Urine Cytology Findings of Polyomavirus Infections

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