Figure 3. Soluble GST-CRT blocks TSP and hep I-mediated focal adhesion disassembly.

Figure 3

Soluble GST-CRT blocks TSP and hep I-mediated focal adhesion disassembly. BAE cells were grown on coverslips until near confluence. Hep I (1 nM) (A) or TSP (167 nM) (B) were incubated in the absence or the presence of increasing concentrations of GST-CRT (closed symbols) for 20 minutes before addition to washed BAE cells. Cells were then incubated with these proteins for 1 hour at 37°C, fixed and scored for the number of cells positive for focal adhesions as determined by IRM. Replicate coverslips were also incubated with increasing concentrations of GST-CRT (open symbols) in the absence of hep I (A) or TSP (B). Results are expressed as the mean percent of cells positive for focal adhesions ± S.D., n = 3.Reprinted with permission from: Goicoechea S, Orr AW, Pallero MA, Eggleton P, Murphy-Ullrich JE 2000;275:36358–36368. 2000 The American Society for Biochemistry and Molecular Biology, Inc.

From: Cell Surface Calreticulin: Role in Signaling Thrombospondin Anti-Adhesive Activity

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