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Figure 8. Biochemical characterization of mutant CK2β subunits.

Figure 8

Biochemical characterization of mutant CK2β subunits. A) Gel exclusion chromatography of wt CK2β and mutants CK2βP110D and CK2β>V143D. Proteins were chromatographed on a Ultrogel ACA44 column equilibrated in 20mM Tris, HCl pH 7.4, 0.2M NaCl, 2% glycerol. Fractions were collected for adsorbance determination at 280nm. (\0x01) wt CK2β; (\0x01) CK2βP110D (\0x02 )CK2βV143D. B) Gel exclusion chromatography of CK2 holoenzymes reconstituted by combining CK2α subunit with wt and mutated CK2β subunits. Proteins were loaded on a Sephacryl S-200 HR column equilibrated in 20mM Tris. HCl pH 7.4, 0.2M NaCl, 2% glycerol. Fractions were collected for protein kinase activity determinations. (\0x01 ) wt CK2β; (\0x01 ) CK2βP110D; (\0x02 ) CK2βV143D Elution volumes for marker proteins of known molecular mass were determined separately. (unpublished results from our group).

From: A Zinc Ribbon Motif Is Essential for the Formation of Functional Tetrameric Protein Kinase CK2

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