Figure 3. a) Fluorescent photograph of oligodendrocytes present in the spinal cord visualized with carbonic anhydrase II immunostaining.

Figure 3

a) Fluorescent photograph of oligodendrocytes present in the spinal cord visualized with carbonic anhydrase II immunostaining. Scale bar = 50 μm. b) High magnification photograph shows two oligodendrocytes stained by using carbonic anhydrase enzyme histochemistry. Note the long, parallel branching processes. Scale bar = 10 μm. c) Distribution of myelinated tracts in the dorsal part of the spinal cord. Myelin sheaths are immunostained for myelin basic protein (MBP), a major protein present in normal myelin. DF: dorsal funiculus. Scale bar = 50 μm. d) Ramified microglial cells (arrows) in the grey matter of intact spinal cord. Note the faint staining and fine ramifications. Scale bar = 20 μm. Immunostaining to complement receptor type 3 (OX-42). e) Astrocytes in the intact spinal cord visualized by immunostaining to glial fibrillary acidic protein (GFAP). Scale bar = 20 μm. f ) Reactive astroglial cells in an injured spinal cord (seven days after injury). Note the increased GFAP content and the thicker processes of the reactive cells (arrows). Scale bar = 20 μm. g) Low magnification photograph of the ventral part of spinal cord. The Schwann cells are immunostained with the Rat-401 antibody which is specific to Schwann cells in the adult CNS. Note that no immunostaining can be seen in the spinal cord (sc) only in the attached ventral roots (vr). Scale bar = 20 μm.

From: Anatomy and Physiology of the Spinal Cord

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