Figure 10. Pigment cells generate multiphenotypic clonal progeny in vitro.

Figure 10

Pigment cells generate multiphenotypic clonal progeny in vitro. A) Culture of a single melanocyte from quail embryonic epidermis 4 hours after clonal seeding. B) After 13 days in the presence of endothelin-3 (ET3), the melanocyte has generated a large colony with a highly pigmented core. C) Detailed view of (B)—the periphery of the colony comprises both unpigmented and pigmented cells. D,E) Phenotypic analysis show that colony contains (D) melanoblasts and glial cells (immunoreative to MelEM and SMP markers)147 as well as (E) myofibroblasts (expressing α-smooth muscle actin αSMA). Bars: 100 μm in (A) and (E), 200 μm in (C) and (D), 1 mm in (B).

From: The Contribution of the Neural Crest to the Vertebrate Body

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