Figure 3. Colon cancer cell-derived TGF-β transdifferentiates colon fibroblasts into pro-invasive myofibroblasts.

Figure 3

Colon cancer cell-derived TGF-β transdifferentiates colon fibroblasts into pro-invasive myofibroblasts. A) Concentration-dependent effect of a 7-day pretreatment with recombinant (r)TGF-β1 (abscissa) on the expression of α-SMA (diamonds and left side ordinate) and on the pro-invasive activity of the fibroblasts on HCT-8/E11 colon cancer cells (bars and right side ordinate; *, significantly different from the pro-invasive activity of untreated fibroblasts in three experiments). B) Western blot of total lysates from fibroblasts treated (+) or not (-) as indicated during 7 days, immunoprobed for a-SMA followed by stripping and reprobing for γ-tubulin as a loading control. C) Time-dependent effect of pretreatment with 1 ng/ml rTGF-β1 (abscissa) on the pro-invasive activity of the fibroblast (fib) conditioned medium (CM); *, significantly different from the pro-invasive activity of untreated fibroblasts in three experiments.

From: Pro-Invasive Molecular Cross-Signaling between Cancer Cells and Myofibroblasts

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