FIGURE 14.5. Top: Representative 2D-DIGE gels illustrating fractionated proteins from NCM in a comparison for the 1-hour (control vs.

FIGURE 14.5

Top: Representative 2D-DIGE gels illustrating fractionated proteins from NCM in a comparison for the 1-hour (control vs. 1 hour; left) and 3-hour (control vs. 3 hours; right) groups. Control samples were labeled with Cy3 (green) while experimental samples were labeled with Cy5 (red). Internal control samples were labeled with Cy2 (blue, not shown). Bottom: Coomassie blue-stained gels illustrating differentially regulated spots (arrows) in the 1-hour (left) and 3-hour (right) conditions, as revealed by quantitative and statistical analyses with DeCyder software. All differentially regulated spots underwent protein fingerprinting by mass-spectrometry. Source: Figure from Pinaud, R., Osorio, C, Alzate, O. and Jarvis, E. D. (2008) Profiling of experience-regulated proteins in the songbird auditory forebrain using quantitative proteomics, Eur J Neurosci 27:1409–22.

From: Chapter 14, Proteomics of Experience-Dependent Plasticity in the Songbird Auditory Forebrain

Cover of Neuroproteomics
Neuroproteomics.
Alzate O, editor.
Boca Raton (FL): CRC Press; 2010.
Copyright © 2010 by Taylor and Francis Group, LLC.

NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.