FIGURE 4.3. Scheme for generating targeted tetracycline-inducible shRNA transgenic mice.

FIGURE 4.3

Scheme for generating targeted tetracycline-inducible shRNA transgenic mice. A caspase-6 (C6) shRNA is inserted in a landing pad cassette resident at the rosa 26 locus of the mouse genome. The recombination is afforded by the action of FLP enzyme (expressed from CAGGS-FLP) acting on resident DNA recombination sites F3 and FRT that flank the DNA cassette. C6 shRNA-positive clones are identified by selection in G418 as a result of expression of the neomycin-resistance gene (neoR) carried in on the recombined insert. SA, splice acceptor; HygR, hygromycin resistance gene. (Slide courtesy of Artemis Pharmaceuticals GmbH.)

From: Chapter 4, Target Validation for Huntington’s Disease

Cover of Neurobiology of Huntington's Disease
Neurobiology of Huntington's Disease: Applications to Drug Discovery.
Lo DC, Hughes RE, editors.
Boca Raton (FL): CRC Press; 2011.
Copyright © 2011 by Taylor and Francis Group, LLC.

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