FIGURE 2.3. Measurements of cAMP concentration after agonist-induced receptor activation.

FIGURE 2.3

Measurements of cAMP concentration after agonist-induced receptor activation. (A) Schematic drawing of the measurement configuration. (B) Coexpression of recombinant 5-HT4 receptors and HvCNG channels, together with the Gs-proteins, lead to a decrease of the activation time constant for the current during 1 sec hyperpolarization to –100 mV in parallel with serotonin application (0.1 μM). This represents an increase in intracellular cAMP-concentration. A repetitive stimulation of the receptor results in a slow decrease of cAMP response. (C) In contrast, coexpression of recombinant 5-HT1A receptors together with the HvCNG channels and Gi-proteins results in increase of the activation time constant during serotonin application (0.1 μM).

From: Chapter 2, Monitoring Receptor-Mediated Changes of Intracellular cAMP Level by Using Ion Channels and Fluorescent Proteins as Biosensors

Cover of Serotonin Receptors in Neurobiology
Serotonin Receptors in Neurobiology.
Chattopadhyay A, editor.
Boca Raton (FL): CRC Press; 2007.
Copyright © 2007, Taylor & Francis Group, LLC.

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