Figure 3. CID2950007 Inhibits Cdc42 association with PAK-PBD.

Figure 3CID2950007 Inhibits Cdc42 association with PAK-PBD

40 nM His-Cdc42 wt was incubated for 20 min at 30°C with various concentrations of GTPγS in the presence of 1 mM EDTA and DMSO alone (lanes 1–3) or 10 μM CID2950007 (lanes 4–6). Reactions were stopped by adding excess Mg2+. The mixture was incubated with 15 μl PAK-PBD beads (Cytoskeleton, Inc.) for 1 h, washed 2 times and active Cdc42 was analyzed by Western blot probing with a mAb directed against Cdc42. Lanes 1 and 4: 100 nM GTPγS; lanes 2 and 5: 500 nM GTPγS; lanes 3 and 6: 1000 nM GTPγS. Lane 7 is a specificity control incubation containing PAK-PBD beads but no added Cdc42.

From: A Potent and Selective Inhibitor of Cdc42 GTPase

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