Synonyms: Southern analysis, Southern blot analysis, Southern blotting, Southern blotting analysis
Molecular genetic testing technique used to detect differences in the lengths of DNA fragments following restriction enzyme digestion (commonly known as RFLPs: restriction fragment length polymorphisms). The lengths of restriction fragments can vary due to mutations occurring between two restriction sites (e.g., large insertions, large deletions, and highly expanded trinucleotide repeats) or within a restriction site (e.g., single base pair changes).
Related Terms: direct DNA analysis; molecular genetic testing; restriction fragment length polymorphism analysis; restriction site; Targeted mutation analysis
