Figure 1. Assay optimization for assay media, incubation period, and MOI.

Figure 1Assay optimization for assay media, incubation period, and MOI

(A) Vero E6 cells were infected with WNV at 0.05 MOI using the 96-well plate format and developed on successive days. Virus cultured in MEM-E produced a higher CPE between day 3 and day 5 than in DMEM. (B) Assay robustness with Z′ factor and inhibition profiles using MPA (positive control) and different amounts of virus in a 384-well plate format. Z′ factor was higher than 0.75 in a 0.01 ~ 1 MOI range. The inhibitory activity of MPA was greater at the lower MOI.

From: A Cell Based Assay for the Identification of Lead Compounds with Anti-Viral Activity Against West Nile Virus

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