Table 4Reagents and Source

Assay (AID)Reagent (Source)
Recombinant p97 protein (produced in the Deshaies Laboratory)
Magnesium Chloride (Fisher, part M33-500)
1M Tris, pH 8.0 (Invitrogen, part T-3038)
0.5M EDTA (Invitrogen, part 15575-025)
Dithiothreitol (Fisher, part BP172-25)
3N Sodium Acetate pH 5.2 (Sigma, part S7899)
ATP (Sigma, part A7699-1G)
Kinase-Glo (Promega, part K1214)
Methanol (Fisher, part A412-4)
DMSO (Acros Organics, part 127790025)
1536-well plates (Greiner, part 789072)
384-well plates (Corning, part 3704)
All as above except replace Recombinant p97 protein (produced in the Deshaies Laboratory) with mutant p97 C522A protein
Ub-GFP Assay (refer to Summary AID TBD)MG132 (Biomol, part PI102-0025)
PBS ( Invitrogen, part 10010-049)
DMEM (Invitrogen, part 10313-039)
FBS (Atlanta Biological, part s115)
Penicillin-Streptomycin (Invitrogen, part 15140-163)
Cycloheximide (Calbiochem, part 239763)
96-Well CELLSTAR® Black μClear Bottom Plates (ISC Bioexpress, part T-3026-16)
BD Falcon Standard Tissue Culture Dishes 100 dia. x 20mm H (Fisher Scientific, part08-772E)
HeLa cells stably expressing Ub-G76V-GFP reporter (obtained from Nico Dantuma (1,2).

From: Probe Report for P97/cdc48 Inhibitors

Cover of Probe Reports from the NIH Molecular Libraries Program
Probe Reports from the NIH Molecular Libraries Program [Internet].

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