Scheme 1. Example qHTS data and classification scheme for assignment of resulting curve-fit data into classes.

Scheme 1Example qHTS data and classification scheme for assignment of resulting curve-fit data into classes

Top, qHTS curve-fit data from AID-361 binned into curve classifications 1-4 based classification criteria. Below, Examples of curves fitting the following classification criteria: Class 1 curves display two asymptotes, an inflection point, and r2 ≥ 0.9; subclasses 1a (blue) vs. 1b (orange) are differentiated by full (>80%) vs. partial (≤ 80%) response. Class 2 curves display a single left-hand asymptote and inflection point; subclasses 2a (blue) and 2b (orange) are differentiated by a max response and r2, >80% and >0.9 or <80% and <0.9, respectively. Class 3 curves have a single left-hand asymptote, no inflection point, and a response >3SD the mean activity of the sample field. Class 4 defines those samples showing no activity across the concentration range. Specifically, for the identification of the Probe series 1, the filtering shown in Figure 3 below was used.

From: A high-throughput screen for pre-mRNA splicing modulators

Cover of Probe Reports from the NIH Molecular Libraries Program
Probe Reports from the NIH Molecular Libraries Program [Internet].

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