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Figure 4. The COPI-Mediated Retrograde Trafficking Pathway is a negative regulator of lipid storage.

Figure 4The COPI-Mediated Retrograde Trafficking Pathway is a negative regulator of lipid storage

(A–F) Drosophila cells with or without oleic acid stained with BODIPY493/503 to detect lipid. Control cells not treated with dsRNA (A and D) or cells incubated with dsRNAs targeting Arf79F (an Arf1 homolog) (B and E) or alphaCop (C and F) are shown. (G) All COPI, and several COPII members as well as additional Arfs, were retested using independent dsRNAs and gave similar results. Results and number of dsRNAs present in the primary screen (including oleic acid) and retests are given. (H) Dose response of Drosophila S3 cells to Exo1 (structure inset) showing the %-activity derived either from the lipid specific signal (filled circles) or the lipid/cell ratio (open circles). Percent activity refers hereby to the changes of lipid storage relative to Triacsin C treatment, which decreases lipid storage by blocking TG synthesis. Increased activity indicates increased lipid storage, which increased with concentration. Scale bar in (A) represents 10. Adopted from Beller et al. 2008. (13)

From: Identification of lipid storage modulators

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Probe Reports from the NIH Molecular Libraries Program [Internet].

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