Figure 5. ES cell nuclear architecture.

Figure 5ES cell nuclear architecture.

A. Chromosome territories. Chromosome 17 territories in mouse ES cell interphase labeled with a specific chromosome paint probe (green); DAPI staining is in blue. B. Sites of transcription. Nascent RNA in interphase nuclei of undifferentiated mouse ES cells is labeled with bromo-uridine (BrU) followed by immunolabeling with anti BrU antibody (red). The nucleoli – the sites of ribosomal RNA transcription – are most conspicuously labeled, shown as two large foci in the center. The remaining red signal marks sites of RNA polymerase II (RNA pol II) transcription. C. Transcription factories. Immunostaining of interphase mouse ES cell nuclei with an antibody specific for the phosphorylated form (on serine 2) of the carboxyl terminal domain (CTD) of RNA pol II marks mRNA transcription sites throughout the entire nucleoplasm (green). Bar = 5 μm.

From: Imaging chromatin in embryonic stem cells

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StemBook [Internet].
Cambridge (MA): Harvard Stem Cell Institute; 2008-.
Copyright: © 2008 Eran Meshorer.

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