Figure 4. Progress towards the elimination of ES nucleus following fusion, as described by Matsumura et al. (Matsumura et al., 2007).

Figure 4Progress towards the elimination of ES nucleus following fusion, as described by Matsumura et al. (Matsumura et al., 2007).

A “Chromosome Elimination Cassette” (CEC) consisting of two oppositely-oriented LoxP sites flanking a GFP transgene is introduced into the ES cell genome in a single copy. Following DNA replication, the introduction of Cre mediates recombination between the CEC's on the two sister chromatids, yielding abnormal chromosomes with either no centromere (nullicentric) or two centromeres (dicentric). During cell division, these abnormal chromosomes are naturally eliminated, thereby removing the ES cell-derived chromosome from the hybrid cell.

From: Inducing pluripotency

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StemBook [Internet].
Cambridge (MA): Harvard Stem Cell Institute; 2008-.
Copyright: © 2008 Kit T Rodolfa.

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