Figure 5-10. Editing by DNA polymerase.

Figure 5-10Editing by DNA polymerase

Outline of the structures of DNA polymerase complexed with the DNA template in the polymerizing mode (left) and the editing mode (right). The catalytic site for the exonucleolytic (E) and the polymerization (P) reactions are indicated. To determine these structures by x-ray crystallography, researchers “froze” the polymerases in these two states, by using either a mutant polymerase defective in the exonucleolytic domain (right), or by withholding the Mg2+ required for polymerization (left).

From: DNA Replication Mechanisms

Cover of Molecular Biology of the Cell
Molecular Biology of the Cell. 4th edition.
Alberts B, Johnson A, Lewis J, et al.
New York: Garland Science; 2002.
Copyright © 2002, Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter; Copyright © 1983, 1989, 1994, Bruce Alberts, Dennis Bray, Julian Lewis, Martin Raff, Keith Roberts, and James D. Watson .

NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.