FIGURE 5.4. Signal parameters and reproducibility of electrochemical signals produced by local application of 5-HT in the CA3 region of the anesthetized mouse.

FIGURE 5.4

Signal parameters and reproducibility of electrochemical signals produced by local application of 5-HT in the CA3 region of the anesthetized mouse. Serotonin (2 pmol, indicated by arrows along the lower abscissa) was applied at ~3 min intervals by pressure-ejection. Shown is the oxidation current converted to micromolar concentration using a calibration factor determined in vitro. Once at least 3 reproducible 5-HT signals were obtained, phosphate buffered saline (PBS, a commonly used vehicle for drugs) was applied 60 s prior to the next application of 5-HT. Note the lack of effect of PBS on the 5-HT signal.

From: Chapter 5, High-Speed Chronoamperometry to Study Kinetics and Mechanisms for Serotonin Clearance In Vivo

Cover of Electrochemical Methods for Neuroscience
Electrochemical Methods for Neuroscience.
Michael AC, Borland LM, editors.
Boca Raton (FL): CRC Press; 2007.
Copyright © 2007, Taylor & Francis Group, LLC.

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