FIGURE 14.8. Amperometric monitoring of release via an artificial fusion pore.


Amperometric monitoring of release via an artificial fusion pore. (a) Amperometric detection of release from a vesicle with a radius of 5 μm, showing prespike feet (arrows), indicating catechol transport through the lipid nanotube or fusion pore. (Scale bar is 80 pA and 500 ms.) (b) Time correlation of vesicle growth, transport of transmitter through the lipid nanotube, and the final stage of exocytosis with amperometric detection. (c) Plots of foot length, (d) foot area, and (e) the ratio of foot area over foot length, all observed with amperometry for vesicles fusing with an artificial cell, at three different pressures used to inflate the vesicles. (f) Schematic model of the factors affecting flow in the vesicle and nanotube of the artificial cell. (Reproduced from Cans, A. S., Wittenberg, N., Karlsson, R., Sombers, L., Karlsson, M., Orwar, O., Ewing, A., Proc. Natl Acad. Sci. U.S.A., 100 (2), 400–404, 2003. With permission.)

From: Chapter 14, Electrochemistry at the Cell Membrane/Solution Interface

Cover of Electrochemical Methods for Neuroscience
Electrochemical Methods for Neuroscience.
Michael AC, Borland LM, editors.
Boca Raton (FL): CRC Press; 2007.
Copyright © 2007, Taylor & Francis Group, LLC.

NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.